SILICA-GEL - AN IDEAL MATERIAL FOR FIELD PRESERVATION OF LEAF SAMPLES FOR DNA STUDIES

SILICA-GEL - AN IDEAL MATERIAL FOR FIELD PRESERVATION OF LEAF SAMPLES FOR DNA STUDIES
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DOI:
10.2307/1222975
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发表时间:
1991-05-01
期刊:
影响因子:
3.4
通讯作者:
HILLS, HH
HILLS, HH
中科院分区:
生物学2区
文献类型:
--
作者:
CHASE, MW;HILLS, HH

文献摘要

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硅胶是一种廉价而可靠的物质,可以保存野外采集的叶片,用于DNA变异的分子研究。解释了其利用的方法,并提出了结果,比较总细胞DNA样品提取的一组新鲜和硅胶干燥样品的相同的物种,以及检查酶切的效率和完整性的DNA从一组现场收集的叶片保存与硅胶。
Silica gels an inexpensive and reliable substance to preserve field-collected leaves for molecular studies of variation in DNA. A method for its utilization is explained, and results are presented, comparing total cellular DNA samples extracted from a set of fresh and silica-gel dried samples of the same species, as well as examining the efficiency of endonuclease restriction and intactness of DNA from of a set of field-collected leaves preserved with silica gel.