Interferon induction and suppression in swine testicle cells by porcine parvovirus and its proteins
Interferon induction and suppression in swine testicle cells by porcine parvovirus and its proteins
复制标题
猪细小病毒及其蛋白对猪睾丸细胞干扰素的诱导和抑制
DOI：
10.1016/j.vetmic.2012.11.032
复制
发表时间：
2013-04-12
影响因子：
3.3
通讯作者：
Cui, Shangjin
中科院分区：
文献类型：
--
作者：
Lin, Wencheng;Qiu, Zheng;Cui, Shangjin
猪细小病毒(PPV)是引起猪繁殖障碍的主要病原，目前影响全球养猪业。虽然PPV早在几年前就被发现了，但人们对它如何克服宿主的先天免疫知之甚少。在这项研究中，我们使用实时定量聚合酶链式反应和荧光素酶报告实验来确定PPV感染是否诱导I型干扰素(干扰素-α和干扰素-β)，以及PPV感染是否阻断双链RNA诱导的细胞培养中干扰素-β启动子的激活。结果表明，PPV不诱生I型干扰素，PPV的NS2蛋白可阻断dsRNA诱导的干扰素-β启动子的激活。(C)2012爱思唯尔B.V.保留所有权利。
Porcine parvovirus (PPV) is a major causative agent of reproductive failure in swine, which currently affects the swine industry worldwide. Although PPV was identified several years ago, little is known about how it overcomes host innate immunity. In this study, we used quantitative real-time PCR and a luciferase reporter assay to determine whether PPV infection induces type I interferon (IFN-alpha and IFN-beta) and whether PPV infection blocks dsRNA-induced IFN-beta promoter activation in cell cultures. The results indicate that PPV does not induce type I interferon and that the NS2 protein of PPV could blocks dsRNA-induced IFN-beta promoter activation. (C) 2012 Elsevier B.V. All rights reserved.