Random amplified polymorphic DNA of mosquito species and populations (Diptera: Culicidae): techniques, statistical analysis, and applications.
Random amplified polymorphic DNA of mosquito species and populations (Diptera: Culicidae): techniques, statistical analysis, and applications.
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蚊子种类和种群（双翅目：蚊科）的随机扩增多态性 DNA：技术、统计分析和应用。
DOI：
10.1093/jmedent/29.6.939
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发表时间：
1992
影响因子：
2.1
通讯作者：
Rai,KS
中科院分区：
文献类型：
--
作者：
Kambhampati,S;Black4th,WC;Rai,KS
描述了一种基于聚合酶链反应（PCR）的方法，用于鉴定和区分蚊子物种和种群。首先由威廉姆斯等人（1990）描述的该方法使用任意DNA序列的单个10个碱基长的引物，并导致被称为随机扩增多态性DNA（RAPD）的DNA的随机片段的扩增。我们希望确定是否RAPD蚊子DNA可以用于种和种群的分化，鉴定未知标本，并重建的亲缘关系。蚊子DNA的RAPD扩增导致一系列不同长度的DNA片段。大多数扩增的片段是唯一的个人，但是，我们的数据表明，在每一个的五个物种ofAedesexamined，一些片段是物种特异性的，并存在于该物种的所有个人。这使我们能够为五个物种中的每一个得出诊断谱。最近邻分析的所有扩增的DNA片段区分物种之间的多元基础上。几个个体ofAedes albopictus（Skuse），包括在分析中的“未知数”，被正确地确定为属于Ae。UPGMA聚类的存在和不存在的数据，使不同的Aedes种以及Ae的不同群体的分离。RAPD技术在昆虫学上的应用主要包括建立物种鉴定的诊断图谱和区分同种种群。
A polymerase chain reaction (PCR)-based method is described for the identification and differentiation of mosquito species and populations. The method, described first by Williams et al. (1990), employs single 10 base-long primers of arbitrary DNA sequence and results in the amplification of random segments of DNA known as random amplified polymorphic DNA (RAPD). We wished to determine if RAPD of mosquito DNA could be used for the differentiation of species and populations, identification of unknown specimens, and the reconstruction of phylogeny. RAPD of mosquito DNA results in the amplification of a series of DNA fragments of varying length. Most amplified fragments are unique to an individual; however, our data indicated that in each of the five species ofAedesexamined, some fragments are species-specific and are present in all individuals of that species. This enabled us to derive a diagnostic profile for each of the five species. A nearest-neighbor analysis of all the amplified DNA fragments discriminated among species on a multivariate basis. Several individuals ofAedes albopictus(Skuse), included in the analysis as “unknowns,” were correctly identified as belonging toAe. albopictus.UPGMA clustering of presence–absence data enabled the separation of differentAedesspecies as well as different populations ofAe. albopictus.The entomological applications of RAPD include the construction of diagnostic profiles for species identification and differentiation among conspecific populations.