Cholecystokinin and gastric distension activate oxytocinergic cells in rat hypothalamus.
Cholecystokinin and gastric distension activate oxytocinergic cells in rat hypothalamus.
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胆囊收缩素和胃扩张激活大鼠下丘脑中的催产素能细胞。
DOI：
10.1152/ajpregu.1987.253.4.r661
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Verbalis,JG
中科院分区：
文献类型：
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作者：
Renaud,LP;Tang,M;McCann,MJ;Stricker,EM;Verbalis,JG
全身给予胆囊收缩素八肽 (CCK-8) 会导致大鼠催产素 (OXY) 循环水平突然增加，但不会导致加压素 (VP) 水平突然增加。本研究确定CCK-8是否选择性刺激戊巴比妥麻醉的雄性大鼠下丘脑视上核中分泌OXY的神经元。根据逆向识别的神经分泌神经元的放电模式和对外周压力感受器激活的反应，将其分为假定的 OXY 和 VP 分泌细胞。在研究的 36 个 OXY 分泌细胞中，有 30 个表现出在通过静脉内或腹膜内注射施用 CCK-8 后 2 分钟内放电频率增加 50-200%，而研究的 8 个 VP 分泌神经元中没有一个被激活。相关实验中，用4-10ml空气给大鼠胃内气球充气； 22 个 OXY 分泌神经元中的 20 个表现出放电频率突然且容易可逆的增加，而 17 个 VP 分泌细胞中只有 2 个被激活。在留置胃插管的未麻醉大鼠中，胃扩张同样会升高血浆氧水平。连同之前的发现，即胃迷走神经切断术减弱了 CCK-8 对 OXY 释放的影响，这些观察结果清楚地证明了大鼠胃和神经垂体之间存在敏感的神经联系。
Systemic administration of cholecystokinin octapeptide (CCK-8) prompts an abrupt increase in circulating levels of oxytocin (OXY) but not vasopressin (VP) in rats. The present study determined whether CCK-8 selectively stimulated OXY-secreting neurons in the hypothalamic supraoptic nucleus of pentobarbital-anesthetized male rats. Antidromically identified neurosecretory neurons were categorized into putative OXY- and VP-secreting cells on the basis of their firing patterns and response to peripheral baroreceptor activation. Of 36 OXY-secreting cells studied, 30 demonstrated a 50-200% increase in firing frequency within 2 min of administering CCK-8 by intravenous or intraperitoneal injection, whereas none of the eight VP-secreting neurons studied was activated. In related experiments, 4-10 ml of air were used to inflate an intragastric balloon in rats; 20 of 22 OXY-secreting neurons displayed an abrupt and readily reversible increase in firing frequency, whereas only 2 of 17 VP-secreting cells were activated. Gastric distension similarly elevated plasma OXY levels in unanesthetized rats with indwelling gastric cannulas. Together with previous findings that the effects of CCK-8 on OXY release were attenuated by gastric vagotomy, these observations clearly demonstrate the existence of a sensitive neural link between the stomach and the neurohypophysis in the rat.