Efficient bioconversion of l-threonine to 2-oxobutyrate using whole cells of Pseudomonas stutzeri SDM.
Efficient bioconversion of l-threonine to 2-oxobutyrate using whole cells of Pseudomonas stutzeri SDM.
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DOI：
10.1016/j.biortech.2012.01.123
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发表时间：
2012-04
影响因子：
11.4
通讯作者：
Wen Zhang;Chao Gao;Bin Che;Cuiqing Ma;Zhaojuan Zheng;Tong Qin;P. Xu
中科院分区：
文献类型：
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作者：
Wen Zhang;Chao Gao;Bin Che;Cuiqing Ma;Zhaojuan Zheng;Tong Qin;P. Xu
2-氧代丁酸（2-OBA）是一种重要的中间体，在医药和化工领域有着广泛的应用.工业发酵产物l-苏氨酸可作为生产2-OBA的合适起始原料。在本研究中，证实施氏假单胞菌SDM的全细胞具有良好的将l-苏氨酸转化为2-OBA的能力。对L-苏氨酸生物转化生产2-OBA的条件进行了优化。以9.2g菌体干重l-1的SDM菌株全细胞为生物催化剂，在6 h内从30 g l-1 - 1的l-苏氨酸转化为高浓度（25.6g l-1）的2-OBA，摩尔转化率高达99.6%。
2-Oxobutyrate (2-OBA) is an important intermediate with many applications in the drug and chemical industries. l-Threonine, an industrial fermentation product, could be used as a suitable starting material for the 2-OBA production. In this study, whole cells of Pseudomonas stutzeri SDM were confirmed to possess a good ability to convert l-threonine into 2-OBA. The bioconversion conditions were optimized for the 2-OBA production from l-threonine. Using 9.2g dry cell weight l−1of whole cells of strain SDM as biocatalyst, the biocatalytic process produced 2-OBA at a high concentration (25.6gl−1) with a high molar conversion rate (99.6%) at 6h from 30g1−1of l-threonine.