Development of AFEX-based consolidated bioprocessing on wheat straw for biohydrogen production using anaerobic microflora
Development of AFEX-based consolidated bioprocessing on wheat straw for biohydrogen production using anaerobic microflora
复制标题
开发基于 AFEX 的小麦秸秆综合生物处理技术，利用厌氧微生物生产生物氢
DOI：
10.1016/j.ijhydene.2013.04.068
复制
发表时间：
2013-11-22
影响因子：
7.2
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Cao, Guang-Li;Xia, Xun-Feng;Yang, Qian
研究了氨纤维膨胀（APEX）预处理小麦秸秆（WS）的强化生物处理（CBP）制氢工艺。结果表明，腐木屑是一种很好的天然-微生物混合种源，通过富集处理可以提高接种物的产氢能力。为了提高WS的消化率，对APEX预处理条件进行了研究。最佳条件确定为100 ℃，1：1（w/w）氨与生物质负载，和30分钟停留时间。用AFEX预处理的WS比未处理的WS的H-2产率高67.8%。此外，来自APEX预处理的降解产物产生的氢发酵没有影响，如果控制低于1.5%的底物加载当量。此外，最大的AFEX-WS转化率和H-2产量达到0.8%的底物负荷。这项工作表明，CBP提供了一个有前途的平台，直接和成本效益的纤维素氢生产相比，单独的水解和发酵（SHF）和同步糖化和发酵（SSF）。皇冠版权所有（C）2013，氢能出版物，有限责任公司。由爱思唯尔有限公司出版。保留所有权利。
Consolidated bioprocessing (CBP) of wheat straw (WS) pretreated via ammonia fiber expansion (APEX) for hydrogen production was discussed in this study. Results indicate that rotted wood crumb is a good mixed natural-microflora seed source for biohydrogen production from WS. The hydrogen-producing capability of the inoculum could be improved by an enrichment treatment. In order to enhance the digestibility of WS, APEX pretreatment conditions were investigated. The optimal conditions were determined to be 100 degrees C, 1:1 (w/w) ammonia to biomass loading, and 30min residence time. The yield of H-2 was 67.8% higher using AFEX-pretreated WS than using untreated material. Moreover, degradation products derived from APEX pretreatment exerted no impact on hydrogen fermentation if controlled below 1.5% substrate-loading-equivalent. In addition, the maximum AFEX-WS conversion and H-2 production were achieved at substrate loading of 0.8%. This work indicates that CBP offers a promising platform for direct and cost-effective cellulosic hydrogen production compared to separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). Crown Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.