Enzymatic Degradation of Polyethylene Terephthalate Plastics by Bacterial Curli Display PETase
Enzymatic Degradation of Polyethylene Terephthalate Plastics by Bacterial Curli Display PETase
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DOI：
10.1021/acs.estlett.2c00332
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发表时间：
2022-06
期刊：
影响因子：
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通讯作者：
Baotong Zhu;Quanhui Ye;Yoonjoo Seo;Na Wei
中科院分区：
文献类型：
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作者：
Baotong Zhu;Quanhui Ye;Yoonjoo Seo;Na Wei
聚对苯二甲酸乙二醇酯（PET）的广泛生产和使用产生了大量的塑料垃圾，对环境和人类造成了潜在的威胁。相对于传统的化石来源的生产工艺，酶生物催化是一种很有前途的绿色化学替代品，可以实现塑料废物的处理和回收。在这项工作中，我们创造了一种生物催化剂，BIND-PETase，通过基因工程的大肠杆菌卷曲与功能性的PETase酶，用于PET塑料的生物催化降解。在各种反应条件下，BIND-PETase均可降解PET，产生浓度大于3000 μM的降解产物。考察了pH、温度、塑料基体质量载荷、表面活性剂添加量等关键反应参数对反应的影响。BIND-PETase可重复使用用于PET降解，在4°C和室温下保存30天保持稳定，没有明显的酶活性损失（学生测试，p> 0.05）。值得注意的是，BIND-PETase可以降解废水流出基质中的PET微塑料。此外，BIND-PETase可以在环境条件下解聚高结晶的消费后PET废料，降解效率为9.1%。该研究为开发环境友好型生物催化方法解决塑料降解和回收挑战提供了新的前景。
The extensive production and use of polyethylene terephthalate (PET) have generated an enormous amount of plastic waste, which potentially threatens the environment and humans. Enzyme biocatalysis is a promising green chemistry alternative, relative to the conventional fossil-derived production process, to achieve plastic waste treatment and recycling. In this work, we created a biocatalyst, BIND-PETase, by genetically engineering the curli of anEscherichia colicell with a functional PETase enzyme for biocatalytic degradation of PET plastics. BIND-PETase could degrade PET to generate degradation products at the concentration level of greater than 3000 μM under various reaction conditions. The effects of key reaction parameters, including pH, temperature, plastic substrate mass load, and surfactant addition were characterized. BIND-PETase was reusable for PET degradation and remained stable with no significant enzyme activity loss when stored at both 4 °C and room temperature for 30 days (Student’sttest,p> 0.05). Notably, BIND-PETase could enable the degradation of PET microplastics in wastewater effluent matrix. Moreover, BIND-PETase could depolymerize highly crystalline postconsumer PET waste materials under ambient conditions with degradation efficiency of 9.1% in 7 days. This study provides a new horizon for developing environmentally friendly biocatalytic approaches to solve the plastic degradation and recycling challenge.