Antibacterial effects of methanolic and ethanolic leaf extract of Medlar (Mespilus germanica) against bacteria isolated from hospital environment
Antibacterial effects of methanolic and ethanolic leaf extract of Medlar (Mespilus germanica) against bacteria isolated from hospital environment
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枸杞子（Mespilus germanica）甲醇和乙醇叶提取物对医院环境分离细菌的抗菌作用
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
V. Sayadi
中科院分区：
文献类型：
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作者：
S. A. Asbchin;M. Safari;H. Moradi;V. Sayadi
背景：医院感染中最重要的病原体是患者体内的微生物。90%的医院感染是由细菌引起的。枸杞子是一种具有治疗作用的药用植物，历来受到重视。本研究的目的是评价枸杞叶乙醇提取物和乙醇提取物对医院环境细菌的抑菌作用。材料和方法：本实验从伊朗伊拉姆的沙希德·穆斯塔法·霍梅尼医院获取医院内细菌。枸杞叶提取液采用索氏提取法。采用纸片扩散法评价抑菌效果，采用肉汤微量稀释法测定最低抑菌浓度和最低杀菌浓度。结果：从医院分离出铜绿假单胞菌2株，金黄色葡萄球菌3株，大肠埃希菌5株。结果表明，枸杞叶乙醇提取物对铜绿假单胞菌和4株金黄色葡萄球菌的生长均有抑制作用，对除E4菌株外的所有菌株均有抑制作用。最大抑菌活性是对E2菌株，其周围带直径为19.67 mm。对P_1、P_2、P_3和E_2、E_3、E_5、S_1、S_2和S_3三种菌株的最小抑菌浓度均为125 mg/ml。结论：枸杞叶乙醇提取物对引起医院感染的细菌具有明显的抗菌活性，可用于控制感染性疾病。
Background: The most important pathogen in nosocomial infections are microorganisms of the patient's body. Ninety percent of nosocomial infections caused by bacteria. Medlar is a medicinal plant with therapeutic effects which has been emphasized historically. The aim of this study was to evaluate the antibacterial effects of methanolic and ethanolic leaf extract of medlar against bacteria isolated from hospital environment. Materials and Methods: In this experimental study, the nosocomial bacteria were obtained from shahid Mostafa Khomeini hospital, Ilam, Iran. Soxhlet extraction method was used for medlar leaf extract. Disk diffusion method was used to evaluate the antimicrobial effect and broth microdilution method was used to determine the minimum inhibitory concentration and minimum bactericidal concentration. Results: Two strains of Pseudomonas aeruginosa, three strains of Staphylococcus aureus and five strains of Escherichia coli were isolated from hospital. The results showed that the methanolic extract of Medlar leaf inhibited the growth of Pseudomonas aeruginosa strains and four strains of Staphylococcus aureus and also inhibited the growth of all strains of Escherichia coli strains except E4 strain. The maximum antimicrobial activity was against E2 strain which zone diameter around it was 19.67 millimeters. Quantities of minimum inhibitory concentration for all three strains of P1, P2 and P3 and E2, E3, E5, S1, S2 and S3 strains was equal to 125 mg/ml. Conclusion: Medlar leaf methanolic extract possesses significant antibacterial activity against bacteria causing nosocomial infections and so this extract can be considered in the control of infectious diseases.