FoxO1 represses LXRα-mediated transcriptional activity of SREBP-1c promoter in HepG2 cells
FoxO1 represses LXRα-mediated transcriptional activity of SREBP-1c promoter in HepG2 cells
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DOI：
10.1016/j.febslet.2010.09.027
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发表时间：
2010-10-22
期刊：
影响因子：
3.5
通讯作者：
Fang, Fude
中科院分区：
文献类型：
--
作者：
Liu, Xiaojun;Qiao, Aijun;Fang, Fude
最近的研究表明FoxO 1调节SREBP-1c的表达，但具体机制尚不清楚。我们的研究结果表明，FoxO 1抑制SREBP-1c启动子在HepG 2细胞的转录活性。这种抑制不依赖于FoxO 1与SREBP-1c启动子的结合，但LXR反应元件（LXREs）对这种现象至关重要。此外，FoxO 1还强烈抑制LXR α介导的SREBP-1c启动子的转录升高。电泳迁移率变动分析和染色质免疫沉淀进一步表明FoxO 1抑制LXR α与SREBP-c启动子中LXRE结合的能力。胰岛素可部分缓解FoxO 1介导的SREBP-1c启动子活性抑制。(C)2010年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Recent studies have demonstrated that FoxO1 modulates the expression of SREBP-1c, but the exact mechanism remains unknown. Our results demonstrate that FoxO1 suppresses the SREBP-1c promoter transcriptional activity in HepG2 cells. This repression was independent of FoxO1 binding to the SREBP-1c promoter, but LXR responsive elements (LXREs) were crucial to this phenomenon. Moreover, FoxO1 also strongly inhibited the LXR alpha-mediated elevated transcription by SREBP-1c promoter. Electrophoretic mobility shift assay and chromatin immuno-precipitation further suggested the ability of FoxO1 to inhibit LXR alpha binding with the LXRE in the SREBP-c promoter. FoxO1-mediated suppression of SREBP-1c promoter activity could be partially alleviated by insulin. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.