Gamma hydroxybutyric acid (GHB) concentrations in humans and factors affecting endogenous production
Gamma hydroxybutyric acid (GHB) concentrations in humans and factors affecting endogenous production
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DOI：
10.1016/s0379-0738(03)00043-4
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发表时间：
2003-04-23
影响因子：
2.2
通讯作者：
Elliott, SP
中科院分区：
文献类型：
--
作者：
Elliott, SP
滥用药物γ-羟基丁酸（GHB）的内源性给毒理学家带来了各种解释问题。为了获得人体内是否存在内源性伽马--羟丁酸的数据并调查可能影响这一点的任何因素，进行了一项志愿者研究。无GHB受试者的119份尿样和提交进行毒理学分析的25份尿样中的GHB浓度显示，最大尿GHB浓度为3毫克/升。对提交进行毒理学分析的15份血浆样本进行的分析未发现可测量的伽马--羟丁酸（低于2.5毫克/升）。对一名男性和一名女性志愿者进行的研究显示，每隔一周摄入一次不同的饮食食物组后，个体内出现了显著的不依赖肌酐的波动，总的尿GHB浓度分别在0至2.55毫克/升和0至2.74毫克/升之间。尿液中的浓度似乎不受所研究的特定饮食组的影响，通过气相色谱-火焰离子化检测器（GC-FID）和气相色谱-质谱（GC-MS）测量的浓度进一步支持建议的尿液和血浆解释临界值分别为10和4毫克/升，如果低于这一数值，则无法确定所检测到的伽马--羟丁酸是内源性的还是外源性的。(C)2003爱思唯尔科学爱尔兰有限公司保留所有权利。
The endogenous nature of the drug of abuse gamma hydroxybutyric acid (GHB) has caused various interpretative problems for toxicologists. In order to obtain data for the presence of endogenous GHB in humans and to investigate any factors that may affect this, a volunteer study was undertaken. The GHB concentrations in 119 urine specimens from GHB-free subjects and 25 urine specimens submitted for toxicological analysis showed maximal urinary GHB concentrations of 3 mg/l. Analysis of 15 plasma specimens submitted for toxicological analysis detected no measurable GHB (less than 2.5 mg/l). Studies in a male and female volunteer in which different dietary food groups were ingested at weekly intervals, showed significant creatinine-independent intra-individual fluctuation with overall urine GHB concentrations between 0 and 2.55, and 0 and 2.74 mg/l, respectively. Urinary concentrations did not appear to be affected by the particular dietary groups studied.The concentrations measured by gas chromatography with flame ionisation detection (GC-FID) and gas chromatography with mass spectrometry (GC-MS) lend further support to the proposed urinary and plasma interpretative cut-offs of 10 and 4 mg/l, respectively, where below this it is not possible to determine whether any GHB detected is endogenous or exogenous in nature. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.