Understanding the Role of the Master Regulator XYR1 in Trichoderma reesei by Global Transcriptional Analysis.
Understanding the Role of the Master Regulator XYR1 in Trichoderma reesei by Global Transcriptional Analysis.
复制标题
DOI：
10.3389/fmicb.2016.00175
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Silva RN
中科院分区：
文献类型：
--
作者：
Dos Santos Castro L;de Paula RG;Antoniêto AC;Persinoti GF;Silva-Rocha R;Silva RN
我们定义了丝状真菌里氏木霉纤维素材料降解过程中的转录因子XYR 1的作用。在这方面，我们使用T的Δ xyr 1突变株的RNA-Seq进行了全局转录组分析。reesei与在纤维素、槐糖和葡萄糖作为唯一碳源的存在下生长的亲本菌株QM 9414相比。我们发现5885个基因在三种碳源下差异表达。其中，322个基因在纤维素的存在下上调，而367和188分别在槐糖和葡萄糖中上调。关于在XYR 1直接调控下的基因，30和33分别是纤维素和槐糖所独有的。在Δ xyr 1中调节最多的基因属于碳水化合物活性酶（CAZymes）、转录因子和转运蛋白家族。此外，我们强调属于MFS和ABC转运蛋白家族的转运蛋白的下调。其中，MFS成员大多在纤维素存在下下调。在槐糖和葡萄糖中，这些转运蛋白的表达主要上调。我们的研究结果表明，MFS和ABC转运蛋白可能是新的球员在纤维素降解和他们的作用被证明是碳源依赖。我们的研究结果有助于更好地理解XYR 1调控T. reesei在纤维素材料的存在下，从而潜在地增强其在几个生物技术领域中的应用。
We defined the role of the transcriptional factor—XYR1—in the filamentous fungus Trichoderma reesei during cellulosic material degradation. In this regard, we performed a global transcriptome analysis using RNA-Seq of the Δxyr1 mutant strain of T. reesei compared with the parental strain QM9414 grown in the presence of cellulose, sophorose, and glucose as sole carbon sources. We found that 5885 genes were expressed differentially under the three tested carbon sources. Of these, 322 genes were upregulated in the presence of cellulose, while 367 and 188 were upregulated in sophorose and glucose, respectively. With respect to genes under the direct regulation of XYR1, 30 and 33 are exclusive to cellulose and sophorose, respectively. The most modulated genes in the Δxyr1 belong to Carbohydrate-Active Enzymes (CAZymes), transcription factors, and transporters families. Moreover, we highlight the downregulation of transporters belonging to the MFS and ABC transporter families. Of these, MFS members were mostly downregulated in the presence of cellulose. In sophorose and glucose, the expression of these transporters was mainly upregulated. Our results revealed that MFS and ABC transporters could be new players in cellulose degradation and their role was shown to be carbon source-dependent. Our findings contribute to a better understanding of the regulatory mechanisms of XYR1 to control cellulase gene expression in T. reesei in the presence of cellulosic material, thereby potentially enhancing its application in several biotechnology fields.