Simultaneous optical mapping of transmembrane potential and wall motion in isolated, perfused whole hearts
Simultaneous optical mapping of transmembrane potential and wall motion in isolated, perfused whole hearts
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DOI：
10.1117/1.3630115
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发表时间：
2011-09-01
影响因子：
3.5
通讯作者：
Rogers, Jack M.
中科院分区：
文献类型：
--
作者：
Bourgeois, Elliot B.;Bachtel, Andrew D.;Rogers, Jack M.
心脏传播的光学标测传统上受到运动伪影的阻碍，这主要是由于随着心脏移动，光电探测器与组织配准发生变化。我们开发了一种光学标测技术，可以同时记录离体心脏的电波和机械收缩。这允许从跨膜电位（V（m））记录中去除运动伪影，而不使用机电解偶联剂，并且允许在整个器官水平上研究电和机械事件的相互作用。心脏用电压敏感染料di-4-ANEPPS染色，环形标记物附着在心外膜上。由450 nm和505 nm发光二极管在交替帧上引发的荧光通过装配有605 +/-25 nm发射滤光片的相机以700帧/秒记录。标记位置在软件中跟踪。从由每个移动环包围的像素收集由时间上交错的450和505 nm荧光组成的信号。在去隔行之后，505 nm信号由具有运动伪影的V（m）组成，而450 nm信号是最低电压敏感的并且主要包含伪影。两个信号的比值估计V（m）。使用均匀有限应变来量化由每组3个环包围的组织的变形。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI 10.1117/1.3630115]
Optical mapping of cardiac propagation has traditionally been hampered by motion artifact, chiefly due to changes in photodetector-to-tissue registration as the heart moves. We have developed an optical mapping technique to simultaneously record electrical waves and mechanical contraction in isolated hearts. This allows removal of motion artifact from transmembrane potential (V(m)) recordings without the use of electromechanical uncoupling agents and allows the interplay of electrical and mechanical events to be studied at the whole organ level. Hearts are stained with the voltage-sensitive dye di-4-ANEPPS and ring-shaped markers are attached to the epicardium. Fluorescence, elicited on alternate frames by 450 and 505 nm light-emitting diodes, is recorded at 700 frames/per second by a camera fitted with a 605 +/- 25 nm emission filter. Marker positions are tracked in software. A signal, consisting of the temporally interlaced 450 and 505 nm fluorescence, is collected from the pixels enclosed by each moving ring. After deinterlacing, the 505 nm signal consists of V(m) with motion artifact, while the 450 nm signal is minimally voltage-sensitive and contains primarily artifacts. The ratio of the two signals estimates V(m). Deformation of the tissue enclosed by each set of 3 rings is quantified using homogeneous finite strain. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3630115]