Investigating protein translocation in the presence of an electrolyte concentration gradient across a solid‐state nanopore
Investigating protein translocation in the presence of an electrolyte concentration gradient across a solid‐state nanopore
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研究固态纳米孔中存在电解质浓度梯度时的蛋白质易位
DOI：
10.1002/elps.202100346
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发表时间：
2022
期刊：
影响因子：
2.9
通讯作者：
Kim, Min Jun
中科院分区：
文献类型：
--
作者：
Saharia, Jugal;Bandara, Y. M. Nuwan D. Y.;Kim, Min Jun
电解质化学在分析物通过纳米孔的输运特性中起着重要作用。在此，我们报道了不对称LiCl盐浓度下，人血清转铁蛋白（hSTf）在正向（Ctrans/Ccis< 1）或负化学梯度（Ctrans/Ccis> 1）下的易位特性。对于正化学梯度，花楸苷浓度固定在4 M，对于负化学梯度，花楸苷浓度固定在0.5 M，而对于正和负梯度类型，花楸苷浓度在0.5到4 M之间变化，分别导致6种不同的构型。在正化学梯度条件下，至少在一个电压极性下，在所有六种配置中都观察到易位，而在负梯度条件下，在任何极性下都没有观察到任何事件的死浓度。Li+和Cl−离子的通量及其产生的阳离子或阴离子富集区，以及电泳和电渗透运输方向的相互作用，将决定hSTf是否可以穿过孔隙。
Electrolyte chemistry plays an important role in the transport properties of analytes through nanopores. Here, we report the translocation properties of the protein human serum transferrin (hSTf) in asymmetric LiCl salt concentrations with either positive (Ctrans/Ccis< 1) or negative chemical gradients (Ctrans/Ccis> 1). Thecisside concentration was fixed at 4 M for positive chemical gradients and at 0.5 M LiCl for negative chemical gradients, while thetransside concentration varied between 0.5 to 4 M which resulted in six different configurations, respectively, for both positive and negative gradient types. For positive chemical gradient conditions, translocations were observed in all six configurations for at least one voltage polarity whereas with negative gradient conditions, dead concentrations where no events at either polarity were observed. The flux of Li+and Cl−ions and their resultant cation or anion enrichment zones, as well as the interplay of electrophoretic and electroosmotic transport directions, would determine whether hSTf can traverse across the pore.