No effects of human ghrelin on cardiac function despite profound effects on body composition in a rat model of heart failure
No effects of human ghrelin on cardiac function despite profound effects on body composition in a rat model of heart failure
复制标题
DOI：
10.1016/j.ijcard.2008.06.094
复制
发表时间：
2009-11-01
影响因子：
3.5
通讯作者：
Springer, Jochen
中科院分区：
文献类型：
--
作者：
Akashi, Yoshihiro J.;Palus, Sandra;Springer, Jochen
背景资料：Ghrelin，被观察到有治疗潜力的严重慢性心力衰竭（CHF）和心脏恶病质的基础上抗恶病质和cardioprotective effects.Methods：我们进行了两项研究，以评估人ghrelin对食物摄入量的影响，体重和身体成分，以及心脏功能在CHF大鼠模型。研究1（通过泵输注50或500 nmole/kg/d生长素释放肽）侧重于食物摄入和身体组成，研究2（通过皮下注射50或100 nmole/kg/d生长素释放肽（每日3次））侧重于心脏功能，因为在研究1中观察到缺乏心脏效应。在这两项研究中，心肌梗死是由LAD结扎引起的。在手术后第28天，将大鼠随机化并用ghrelin或安慰剂治疗4周。结果：在研究1中，用高剂量Ghrelin治疗的CHF大鼠显示体重增加（+25%，p < 0.05），而在研究2中，体重增加（+25%，p < 0.05），并且在研究3中，体重增加（+25%，p < 0.05），而在研究4中，体重增加（+25%，p < 0.05），而在研究4中，体重增加（+25%，p < 0.05）。
Background: Ghrelin, was observed to have treatment-potential for severe chronic heart failure (CHF) and cardiac cachexia based on anti-cachectic and cardio-protective effects.Methods: We performed two studies to assess the effects of human ghrelin on food intake, body weight and body composition, as well as heart function in a rat model of CHF. Study-1 (50 or 500 nmole/kg/d ghrelin by pump infusion) was focused on food intake and body composition, study-2 (50 or 100 nmole/kg/d ghrelin by subcutaneous injection (3-times daily) was focused on heart function due to a lack of cardiac effects observed in study-1. In both studies, myocardial infarction was induced by LAD ligation. On day 28 after surgery, rats were randomized and treated with ghrelin or placebo for 4 weeks. Food intake (study-1), body composition (NMR) cardiac function (echocardiography and invasive hemodynamics (study-2 only) were assessed.Results: In study-1, CHF rats treated with high dose ghrelin showed an increase in body weight (+25%, p