Human oocyte cryopreservation: new perspectives regarding oocyte survival
Human oocyte cryopreservation: new perspectives regarding oocyte survival
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DOI：
10.1093/humrep/16.3.411
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发表时间：
2001-03-01
影响因子：
6.1
通讯作者：
Flamigni, C
中科院分区：
文献类型：
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作者：
Fabbri, R;Porcu, E;Flamigni, C
人卵母细胞冷冻保存的成功取决于可能影响卵母细胞解冻后存活的形态学和生物物理因素，已经进行了各种冷冻保存人卵母细胞的尝试，但结果却截然相反，因此一些因素，如卵丘的存在与否、冷冻溶液中的蔗糖浓度以及冷冻保护剂的暴露时间等因素对人卵母细胞存活的影响 解冻后的研究，采用慢速冷冻-快速解冻程序，将卵母细胞冷冻保存在添加有蔗糖的1,2-丙二醇中。解冻后，通过胞浆内单精子注射（ICSI）对卵母细胞进行授精，并记录授精和随后的胚胎发育的结果，机械去除部分卵丘冷冻保存的卵母细胞与酶法完全去除卵丘冷冻保存的卵母细胞相比，解冻后冷冻存活率没有差异（56%）。 (53%)，相反，当冷冻溶液中蔗糖浓度加倍(0.2 mol/l)时冷冻保存卵母细胞，存活率显着更高，当蔗糖浓度增加三倍(0.3 mol/l)时，存活率甚至更高（60 vs 82% P < 0.001）。此外，在降低温度之前较长的冷冻保护剂暴露时间（从 10.5 至 15 分钟）可显着提高卵母细胞的存活率 (P < 0.005)。胞浆内单精子注射产生了良好的解冻卵母细胞受精率（57％）和高胚胎卵裂率（91％），并且观察到令人满意的胚胎形态（I级和II级胚胎分别为14％和34％）。
The success of human oocyte cryopreservation depends on morphological and biophysical factors that could influence oocyte survival after thawing, Various attempts to cryopreserve human oocytes have been performed with contrasting results, Therefore the effect of some factors, such as the presence or absence of the cumulus oophorus, the sucrose concentration in the freezing solution and the exposure time to cryoprotectants, on human oocyte survival after thawing were investigated, The oocytes were cryopreserved in 1,2-propanediol added with sucrose, using a slow-freezing-rapid-thawing programme. After thawing, the oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and the outcomes of insemination and subsequent embryo development were also recorded, The post-thaw cryosurvival rate was not different for the oocytes cryopreserved with their cumuli partially removed mechanically (56%) when compared with those cryopreserved with their cumuli totally removed enzymatically (53%), On the contrary, a significantly higher survival rate was obtained when the oocytes were cryopreserved in the presence of a doubled sucrose concentration (0.2 mol/l) in the freezing solution and the survival rate was even higher when the sucrose concentration was tripled (0.3 mol/l) (60 versus 82% P < 0.001). Furthermore, a longer exposure time (from 10.5 to 15 min) to cryoprotectants, before lowering the temperature, significantly increased the oocyte survival rate (P < 0.005). Intracytoplasmic sperm injection produced a good fertilization rate (57%) of thawed oocytes and a high embryo cleavage rate (91%) and a satisfactory embryo morphology was observed (14 and 34% for grade I and grade II embryos respectively).