ANTIMICROBIAL ACTIVITY OF DALBERGIA-MELANOXYLON EXTRACTS

ANTIMICROBIAL ACTIVITY OF DALBERGIA-MELANOXYLON EXTRACTS
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DOI:
10.1016/0378-8741(93)90057-c
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发表时间:
1993-10-01
影响因子:
5.4
通讯作者:
GAZA, N
GAZA, N
中科院分区:
医学2区
文献类型:
--
作者:
GUNDIDZA, M;GAZA, N

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黑木黄檀树皮的甲醇、柠檬酸、水提取物、二氯甲烷提取物和石油醚提取物的抗菌和抗真菌特性通过使用接种琼脂平板和装有酵母提取物和蔗糖肉汤用于真菌菌丝生长的烧瓶来测定,所述琼脂平板中的孔中放置有提取物。孵育24小时后,测量抑菌圈的直径以进行抗菌测试,7天后,测量菌丝体的干重,并使用不添加提取物的对照计算抑制百分比。结果表明,柠檬酸提取物表现出最强的抗菌活性。乙醇级分显示出显着的抗菌活性,但对真菌没有显着的活性。二氯甲烷提取物对细菌没有表现出活性,但对真菌表现出显着的活性。石油醚馏分没有表现出抗菌活性。
The antibacterial and antifungal properties of the methanol, citric acid, aqueous, dichloromethane and petroleum ether extracts from the bark of Dalbergia melanoxylon were determined by using seeded agar plates with wells into which were placed the extract, and flasks of yeast extract and sucrose broth for mycelial growth of the fungi. After incubation for 24 h, the diameter of the inhibition zone was measured for the antibacterial tests and after 7 days, the dry weight of the mycelia was measured and a percentage of inhibition calculated using controls where no extracts were added. The results obtained showed that the citric acid extract exhibited strongest antimicrobial activity. The ethanol fraction showed significant antibacterial activity but was not significantly active against fungi. The dichloromethane extract exhibited no activity against bacteria but showed notable activity against fungi. The petroleum ether fraction showed no antimicrobial activity.