Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression.
Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression.
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将正常人类 11 号染色体引入维尔姆斯肿瘤细胞系可控制其致瘤表达。
DOI：
10.1126/science.3031816
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Stanbridge,EJ
中科院分区：
文献类型：
--
作者：
Weissman,BE;Saxon,PJ;Pasquale,SR;Jones,GR;Geiser,AG;Stanbridge,EJ
肾母细胞瘤（一种小儿肾母细胞瘤）的发生与11号染色体p13区域的缺失有关。这种缺失的遗传物质的结构和功能是未知的。通过微细胞转移技术将正常人11号染色体导入肾母细胞瘤细胞系，研究了该缺失在恶性转化过程中的作用。这些变异细胞，来自微细胞杂交，在文化中表达类似的转化性状作为亲本细胞系。此外，亲代细胞的几种原癌基因的表达不受该染色体引入的影响。然而，这些细胞在裸鼠中形成肿瘤的能力被完全抑制。转移其他染色体，即X和13，对Wilms肿瘤细胞的致瘤性没有影响。这些研究为11号染色体上存在遗传信息提供了支持，这些遗传信息可以控制肾母细胞瘤细胞的恶性表达。
The development of Wilms' tumor, a pediatric nephroblastoma, has been associated with a deletion in the p13 region of chromosome 11. The structure and function or functions of this deleted genetic material are unknown. The role of this deletion in the process of malignant transformation was investigated by introducing a normal human chromosome 11 into a Wilms' tumor cell line by means of the microcell transfer technique. These variant cells, derived by microcell hybridization, expressed similar transformed traits in culture as the parental cell line. Furthermore, expression of several proto-oncogenes by the parental cells was unaffected by the introduction of this chromosome. However, the ability of these cells to form tumors in nude mice was completely suppressed. Transfer of other chromosomes, namely X and 13, had no effect on the tumorigenicity of the Wilms' tumor cells. These studies provide support for the existence of genetic information on chromosome 11 which can control the malignant expression of Wilms' tumor cells.