Aphidius ervi teratocytes release an extracellular enolase
Aphidius ervi teratocytes release an extracellular enolase
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DOI：
10.1016/j.ibmb.2009.09.005
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发表时间：
2009-11-01
影响因子：
3.8
通讯作者：
Pennacchio, Francesco
中科院分区：
文献类型：
--
作者：
Falabella, Patrizia;Riviello, Lea;Pennacchio, Francesco
我们报告了一个基因的克隆和编码的蛋白质，这是由寄生蜂蚜ervi的畸形细胞释放的宿主蚜虫Acyrthosiphon豌豆的血腔的特征。通过LC-MS/MS鉴定所研究的蛋白质，并且收集的信息用于分离全长cDNA。该基因由3个外显子和2个内含子组成，在成蜂和被寄生的寄主中表达量较高。翻译产物Ae-ENO与昆虫烯醇化酶具有很高的同源性。体内免疫检测实验证明Ae-ENO定位在圆形斑点，存在于畸形细胞和释放在主机血腔。此外，在A. ervi幼虫和宿主胚胎。在昆虫细胞中表达的Ae-ENO在培养基中不分泌，表明Ae-ENO释放的未知途径在畸形细胞中发生。在天然条件下在细菌中产生的重组蛋白是二聚体，具有明显的烯醇化酶活性（K-m = 0.086 +/- 0.017 mM）。烯醇化酶是细胞代谢中众所周知的酶，然而，当存在于细胞外环境中、原核和真核细胞表面上时，其与疾病中的多功能作用相关。在这些情况下，烯醇化酶介导参与病原体和肿瘤细胞侵入组织以及逃避宿主免疫应答的酶的活化。Ae-ENO在主机调节过程中可能发挥的作用进行了讨论，根据这些信息。(C)2009爱思唯尔有限公司保留所有权利。
We report the cloning of a gene and the characterization of the encoded protein, which is released by the teratocytes of the parasitoid Aphidius ervi in the haemocoel of the host aphid Acyrthosiphon pisum. The studied protein was identified by LC-MS/MS, and the gathered information used for isolating the full length cDNA. The corresponding gene was made of 3 exons and 2 introns, and was highly expressed in the adult wasps and in parasitized hosts. The translation product, which was named Ae-ENO, showed a very high level of sequence identity with insect enolases. In vivo immunodetection experiments evidenced Ae-ENO localization in round spots, present in the teratocytes and released in the host haemocoel. Moreover, strong immunoreactivity, was detected on the surface of A. ervi larvae and of host embryos. Ae-ENO expressed in insect cells was not secreted in the medium, indicating the occurrence in the teratocytes of an unknown pathway for Ae-ENO release. The recombinant protein produced in bacteria under native conditions was a dimer, with evident enolase activity (K-m = 0.086 +/- 0.017 mM). Enolase is a well known enzyme in cell metabolism, which, however, is associated with a multifunctional role in disease, when present in the extracellular environment, on the surface of prokaryotic and eukaryotic cells. in these cases, the enolase mediates the activation of enzymes involved in the invasion of tissues by pathogens and tumour cells, and in the evasion of host immune response. The possible role played by Ae-ENO in the host regulation process is discussed in the light of this information. (C) 2009 Elsevier Ltd. All rights reserved.