A 1.5-megabase physical map encompassing the multiple endocrine neoplasia type-1 (MEN1) locus on chromosome 11q13.
A 1.5-megabase physical map encompassing the multiple endocrine neoplasia type-1 (MEN1) locus on chromosome 11q13.
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1.5 兆碱基物理图谱，包含染色体 11q13 上的 1 型多发性内分泌肿瘤 (MEN1) 基因座。
DOI：
10.1006/geno.1996.0612
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发表时间：
1996
期刊：
影响因子：
4.4
通讯作者：
Sawicki,MP
中科院分区：
文献类型：
--
作者：
Wood,TF;Srivatsan,ES;Chakrabarti,R;Ma,GC;Kuan,N;Samara,GJ;Higgins,MJ;Shows,TB;Johnson,CL;Wan,YJ;PassaroJr,EP;Sawicki,MP
连锁分析和杂合性丢失研究表明，导致 1 型多发性内分泌肿瘤 (MEN1) 综合征的基因定位于染色体 11q13 上 D11S427 和 D11S460 之间的一个小间隔。作为克隆该肿瘤抑制基因的第一步，我们的团队是第一个使用酵母人工染色体、细菌人工染色体 (BAC) 和粘粒重叠群物理绘制 MEN1 区域图谱的团队。 1.5 Mb 高分辨率连续图谱从 PYGM 延伸至 D11S460 的 300 kb 端粒。其中，PYGM 和 D11S460 之间的 1.2 Mb 间隔在粘粒和 BAC 中被分离出来，将有助于开发该重要区域的基因组序列和转录图谱。该区域还鉴定出了九个新的序列标记位点 (STS)。物理图谱和 STS 将成为克隆 MEN1 基因的宝贵工具。
Linkage analysis and loss of heterozygosity studies have shown that the gene responsible for the multiple endocrine neoplasia type-1 (MEN1) syndrome localizes to a small interval between D11S427 and D11S460 on chromosome 11q13. As an initial step to clone this tumor suppressor gene, our group is the first to map the MEN1 region physically using yeast artificial chromosome, bacterial artificial chromosome (BAC), and cosmid contigs. The 1.5-Mb high-resolution, contiguous map extends from PYGM to 300 kb telomeric of D11S460. Of this, the 1.2-Mb interval between PYGM and D11S460 is isolated in cosmids and BACs and will be useful for the development of genomic sequences and transcription maps of this important region. Nine new sequence-tagged sites (STS) are also characterized from this region. The physical map and the STSs will be valuable tools for the cloning of the MEN1 gene.