The effect of polymer nanostructure on diffusion of small molecules using tryptophan as a FRET probe
The effect of polymer nanostructure on diffusion of small molecules using tryptophan as a FRET probe
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使用色氨酸作为 FRET 探针聚合物纳米结构对小分子扩散的影响
DOI：
10.1016/j.eurpolymj.2014.06.003
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发表时间：
2015
影响因子：
6
通讯作者：
Moore B
中科院分区：
文献类型：
--
作者：
Moore B
The amino acidL-tryptophan has been converted into a polymerizable monomer which has been incorporated into a range of cross-linked polymeric nanogels prepared by emulsion polymerization. By using time-lapse fluorescence spectroscopy the diffusion time of a small molecule Forster resonance energy transfer (FRET) pair, dansyl amide, into the central nanogel domain has been monitored through the decreased emission of theL-tryptophan FRET donor. In this initial study it has been found that diffusion of the small molecule into the nanogels is affected by altering the synthetic parameters (cross-linking density and co-monomer hydrophobicity). When increasing the cross-linking density of the nanogels the uptake diffusion time increased, while increasing the hydrophobicity of the co-monomer (and consequently lowering the glass-transition temperature (Tg)) caused a decrease in the diffusion time.
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