Aptamers switch on fluorescence of triphenylmethane dyes
Aptamers switch on fluorescence of triphenylmethane dyes
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DOI：
10.1021/ja037994o
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发表时间：
2003-12-03
影响因子：
15
通讯作者：
Tsien, RY
中科院分区：
文献类型：
--
作者：
Babendure, JR;Adams, SR;Tsien, RY
MG和SRB适配体是最初只选择与孔雀石绿或磺胺B结合的短RNA序列，可以极大地增强通常不具有荧光的三苯基甲烷染料的荧光。MG适配体使孔雀石绿（MG）和一种新型刚性衍生物吲哚基孔雀石绿（IMG）的量子产率提高了1000倍。SRB适体使专利蓝V和VF的亮度提高了90倍。这些增强是特异性的，因为MG适体对专利蓝染料没有影响，SRB适体对MG和IMG的影响很小或没有影响。这种短RNA基序的序列特异性荧光标记是在体外和细胞中成像选定RNA的遗传可编码融合标签的第一步。
MG and SRB aptamers, which are short RNA sequences originally selected only for binding to malachite green or sulforhodamine B, can greatly enhance the fluorescence of normally nonfluorescent triphenylmethane dyes. MG aptamer enhances the quantum yields of malachite green (MG) and a novel rigidized derivative, indolinyl malachite green (IMG) by >2000-fold. SRB aptamer brightens patent blue V and VF by >90-fold. These enhancements are specific because MG aptamer has no effect on patent blue dyes and SRB aptamer has little or no effect on MG and IMG. Such sequence-specific fluorescence labeling of short RNA motifs is a first step toward genetically encodable fusion tags for imaging selected RNAs in vitro and in cells.