Infrequent amplification of JUN in hepatocellular carcinoma.
Infrequent amplification of JUN in hepatocellular carcinoma.
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发表时间：
2009-12
影响因子：
2
通讯作者：
Mio Endo;K. Yasui;T. Nakajima;Y. Gen;Kazuhiro Tsuji;O. Dohi;Keika Zen;H. Mitsuyoshi;M. Minami;Y. Itoh;M. Taniwaki;Shinji Tanaka;S. Arii;T. Okanoue;T. Yoshikawa
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作者：
Mio Endo;K. Yasui;T. Nakajima;Y. Gen;Kazuhiro Tsuji;O. Dohi;Keika Zen;H. Mitsuyoshi;M. Minami;Y. Itoh;M. Taniwaki;Shinji Tanaka;S. Arii;T. Okanoue;T. Yoshikawa
目的探讨编码c-Jun蛋白的癌基因JUN在肝细胞癌（HCC）中是否扩增和过表达。材料与方法采用高密度寡核苷酸芯片检测DNA拷贝数畸变。用荧光原位杂交法测定DNA拷贝数。采用实时定量PCR法测定基因组DNA和mRNA含量。结果在JHH-2 HCC细胞系中，在染色体1p32-31区域发现了一种新的扩增，JUN在该区域扩增并过表达。然而，在34例原发性HCC肿瘤中未观察到JUN拷贝数增加（>2倍）。相反，在34个肿瘤中有13个（38%）的JUN表达缺失（<0.5倍），在37个HCC肿瘤中有26个（70%）的JUN表达明显低于非肿瘤肿瘤。结论虽然JUN在JHH-2型HCC细胞中扩增和过表达，但JUN在原发性HCC中扩增和过表达可能很少见。
AIM To determine whether JUN (the oncogene encoding c-Jun protein) is amplified and overexpressed in hepatocellular carcinoma (HCC). MATERIALS AND METHODS DNA copy number aberrations were investigated using a high-density oligonucleotide microarray. DNA copy numbers were determined by fluorescence in situ hybridization. Genomic DNA and mRNA were quantified using real-time quantitative PCR. RESULTS A novel amplification was found at the chromosomal region 1p32-31 in a JHH-2 HCC cell line within which JUN is amplified and overexpressed. However, no copy number gain of JUN (>2-fold) was observed in 34 primary HCC tumors. Rather, a loss of JUN (<0.5-fold) was seen in 13 (38%) out of the 34 tumors and expression of JUN was significantly lower in 26 (70%) out of the 37 HCC tumors compared with their nontumorous counterparts. CONCLUSION Although JUN was amplified and overexpressed in JHH-2 HCC cells, amplification and overexpression of JUN may be rare in primary HCCs.