Resuscitation of dormant Mycobacterium tuberculosis by phospholipids or specific peptides.
Resuscitation of dormant Mycobacterium tuberculosis by phospholipids or specific peptides.
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通过磷脂或特定肽复苏休眠的结核分枝杆菌。
DOI：
10.1006/bbrc.2001.4993
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Sun,Z
中科院分区：
文献类型：
--
作者：
Zhang,Y;Yang,Y;Woods,A;Cotter,RJ;Sun,Z
休眠结核杆菌的存在是结核病治疗的主要问题。结核分枝杆菌的培养上清液先前显示出在体外使休眠的杆菌复苏。在这里，我们报告鉴定的活性成分磷脂和结核蛋白Rv 1174 c。值得注意的是，来自一年龄培养物的不能形成任何菌落的休眠杆菌可以用来源于Rv 1174 c的肽复苏，并形成105- 7菌落/ml。这一发现代表了休眠结核杆菌在体外复苏的第一个明确的证明，并可能对分枝杆菌休眠的研究和设计新的策略，以改善结核病的治疗有意义。
The presence of dormant tubercle bacilli presents a major problem for tuberculosis treatment. The culture supernatant of Mycobacterium tuberculosis was previously shown to resuscitate dormant bacilli in vitro. Here we report identification of active components as phospholipids and a tuberculosis protein Rv1174c. Remarkably, dormant bacilli from a one year old culture which failed to form any colonies could be resuscitated with peptides derived from Rv1174c and formed 105–7colonies/ml. This finding represents the first unambiguous demonstration of resuscitation of dormant tubercle bacilli in vitro and may have implication for the study of mycobacterial dormancy and the design of novel strategies for improved treatment of tuberculosis.