EVALUATION OF THE RAPID CORYNE IDENTIFICATION SYSTEM FOR CORYNEBACTERIUM SPECIES AND OTHER CORYNEFORMS
EVALUATION OF THE RAPID CORYNE IDENTIFICATION SYSTEM FOR CORYNEBACTERIUM SPECIES AND OTHER CORYNEFORMS
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DOI：
10.1128/jcm.30.7.1692-1695.1992
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发表时间：
1992-07-01
影响因子：
9.4
通讯作者：
COYLE, MB
中科院分区：
文献类型：
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作者：
GAVIN, SE;LEONARD, RB;COYLE, MB
根据生产商的说明书，使用177种微生物评价了用于鉴定需氧、非芽孢形成、革兰氏阳性杆菌的快速CORYNE系统。接种生长的重悬液后，将含有20个杯的条带孵育24小时，加入试剂，并将21个生化反应的结果记录为数值曲线。这些菌株由棒状杆菌属的致病种组成，主要是C。白喉杆菌（n = 29）、机会棒杆菌属（Corynebacterium）包括C. jeikeium（n = 75）、公认的非棒状杆菌物种如Gardnerella和Arcanobacterium（n = 51）和疾病控制中心（CDC）棒状杆菌群（n = 22）。24 h后读取的单次检测结果产生了正确的种属水平鉴定结果，其中26种（89.7%）致病种属、64种（85.3%）机会微生物、51种（100%）非棒状杆菌和8种（36.4%）CDC棒状杆菌。补充检测正确鉴定了另外3种致病性分离株（总计100%）和另外4种机会性种属分离株（总计90.6%）。系统未鉴定的15个分离株中有12个属于CDC棒状菌群。6株鉴定错误的菌株中有4株为C. matruchotii，这是不包括在数据库中。该系统是传统生化测试的一个极好的快速替代品。
The Rapid CORYNE system for identification of aerobic, nonsporeforming, gram-positive rods was evaluated according to the manufacturer's instructions with 177 organisms. After inoculation with a heavy suspension of growth, strips containing 20 cupules were incubated for 24 h, reagents were added, and the results of 21 biochemical reactions were recorded as numerical profiles. The strains consisted of pathogenic species of the genus Corynebacterium, primarily C. diphtheriae (n = 29), opportunistic species of Corynebacterium including C. jeikeium (n = 75), recognized species of non-corynebacteria such as Gardnerella and Arcanobacterium (n = 51), and Centers for Disease Control (CDC) coryneform groups (n = 22). Results from single tests read after 24 h yielded correct identifications to species level with no additional tests for 26 (89.7%) of the pathogenic species; 64 (85.3%) of the opportunistic organisms; 51 (100%) of the non-corynebacteria, and 8 (36.4%) of the CDC coryneform groups. Supplemental tests produced the correct identification for three additional pathogenic isolates (100% total) and four additional isolates from the opportunistic species (90.6% total). Twelve of the 15 isolates not identified by the system were in the CDC coryneform groups. Four of the six misidentified and one of the unidentified isolates were C. matruchotii, which was not included in the data base. The system is an excellent rapid alternative to conventional biochemical tests.