Establishment of Genetic Transformation System of Cucumber 'New Jinchun 4'
Establishment of Genetic Transformation System of Cucumber 'New Jinchun 4'
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发表时间：
2010
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通讯作者：
Xu Na;C. Jun;Xia Haiwu;Zhao Yan;Qian Lisheng
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作者：
Xu Na;C. Jun;Xia Haiwu;Zhao Yan;Qian Lisheng
研究了农杆菌介导的黄瓜“新金春4号”遗传转化体系。将黄瓜叶片作为外植体，在MS+1.0 mg L-1 6-BA+0.15 mg L-1 IAA上预培养2 d。经农杆菌（OD600=0.8,pH=5.7）侵染8 min后，添加150 μmol/L乙酰丁香酮（AS），在MS + 1.0 mg L-1 6-BA + 0.15 mg L-1 IAA + 500 mg L-1 Carb + 40 mg L-1 Hyg上共培养2 d。通过瞬时表达和PCR检测，成功地将hpt基因转化为再生植株。
The genetic transformation system of cucumber 'New Jinchun 4' mediated by Agrobacterium tumefaciens were studied.The cucumber leaves as explants were pre-cultured on MS+1.0 mg L-1 6-BA+0.15 mg L-1 IAA for 2 days.After infected by Agrobacterium(OD600=0.8,pH=5.7) for 8 min supplemented with 150 μmol/L acetosyringone(AS),the explants were co-cultured on MS + 1.0 mg L-1 6-BA + 0.15 mg L-1 IAA + 500 mg L-1 Carb + 40 mg L-1 Hyg for 2 days.The hpt gene were transformed successfully to regenerated plantlets by gus instantaneous expression and PCR test.