Direct detection of DNA below ppb level based on thionin-functionalized layered MoS2 electrochemical sensors.
Direct detection of DNA below ppb level based on thionin-functionalized layered MoS2 electrochemical sensors.
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DOI：
10.1021/ac5027786
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发表时间：
2014-11
影响因子：
7.4
通讯作者：
Tanyuan Wang;Ruizhi Zhu;Junqiao Zhuo;Zhiwei Zhu;Y. Shao;Meixian Li
中科院分区：
文献类型：
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作者：
Tanyuan Wang;Ruizhi Zhu;Junqiao Zhuo;Zhiwei Zhu;Y. Shao;Meixian Li
通过在离子液体中超声和梯度离心制备了层状硫化钼-硫离子复合材料。由于DNA很少以单链形式存在，无论是自然存在还是PCR扩增后，由于硫离子与DNA的电化学响应稳定，嵌入和静电相互作用，该复合材料被用于制造双链DNA （dsDNA）电化学生物传感器。在dsDNA浓度范围为0.09 ~ 1.9 ng mL（-1）的线性范围内，适用于单链DNA （ssDNA）的检测。该传感器已应用于健康人血清循环DNA的检测，取得了满意的结果。所构建的DNA电化学生物传感器在生物分析和临床诊断等领域具有潜在的应用前景。此外，本工作还提出了一种基于二硫化钼片构建电化学生物传感器的新方法。
A layered MoS2-thionin composite was prepared by sonicating their mixture in an ionic liquid and gradient centrifugation. Because DNA is rarely present in single-stranded form, either naturally or after PCR amplification, the composite was used for fabrication of a double-stranded DNA (dsDNA) electrochemical biosensor due to stable electrochemical response, intercalation, and electrostatic interaction of thionin with DNA. The linear range over dsDNA concentration from 0.09 ng mL(-1) to 1.9 ng mL(-1) is obtained, and moreover, it is suitable for the detection of single-stranded DNA (ssDNA). The biosensor has been applied to the detection of circulating DNA from healthy human serum, and satisfactory results have been obtained. The constructed DNA electrochemical biosensor shows potential application in the fields of bioanalysis and clinic diagnosis. Furthermore, this work proposes a new method to construct electrochemical biosensors based on MoS2 sheets.