A heterogeneous PNA-based SERS method for DNA detection
A heterogeneous PNA-based SERS method for DNA detection
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DOI：
10.1021/ja0705184
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发表时间：
2007-05-16
影响因子：
15
通讯作者：
Bazan, Guillermo C.
中科院分区：
文献类型：
--
作者：
Fabris, Laura;Dante, Mark;Bazan, Guillermo C.
报道了一种基于 SERS 信号和 PNA 载玻片的 ssDNA 检测的简单方法。与 ssDNA 杂交后，PNA 玻片的表面电荷从中性变为负电。随后用部分聚集的带正电的银纳米颗粒进行处理，导致选择性静电吸附到含有 PNA/ssDNA 双链体的表面上。添加罗丹明 6G 会产生该染料特有的 SERS 信号，可诊断杂交事件。 SERS 图和 AFM 的表征表明，纳米粒子的分布是随机的，并且大约十分之一的聚集位点负责 SERS 光谱。
A simple method for ssDNA detection based on SERS signals and PNA slides is reported. Upon hybridization with ssDNA, the surface charge of the PNA slides changes from neutral to negative. Subsequent treatment with partly aggregated, positively charged silver nanoparticles results in selective electrostatic adsorption onto surfaces containing PNA/ssDNA duplexes. Addition of rhodamine6G gives rise to SERS signals characteristic of this dye, which are diagnostic of the hybridization event. Characterization by SERS maps and AFM reveals that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra.