Simultaneous measurement of urinary and plasma norepinephrine, epinephrine, dopamine, dihydroxyphenylalanine, and dihydroxyphenylacetic acid by coupled-column high-performance liquid chromatography on C8 and C18 stationary phases.
Simultaneous measurement of urinary and plasma norepinephrine, epinephrine, dopamine, dihydroxyphenylalanine, and dihydroxyphenylacetic acid by coupled-column high-performance liquid chromatography on C8 and C18 stationary phases.
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DOI：
10.1016/s0021-9673(01)94652-7
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发表时间：
1987-01
期刊：
影响因子：
--
通讯作者：
C. Benedict
中科院分区：
文献类型：
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作者：
C. Benedict
本文介绍了一种简便、灵敏的测定尿和血浆中去甲肾上腺素、肾上腺素、多巴胺、二羟苯丙氨酸和二羟苯乙酸的方法。该方法包括两个阶段的前端色谱净化，然后耦合微孔高效液相色谱电化学检测。使用苯基硼酸盐代替氧化铝，所有的儿茶酚胺、二羟基苯丙氨酸和二羟基苯乙酸的回收率在80-90%范围内，而没有干扰。测定这些化合物的批间变异系数小于12%。
This paper describes a simple and sensitive method for measuring norepinephrine, epinephrine, dopamine, dihydroxyphenylalanine, and dihydroxyphenylacetic acid in urine and plasma. This method involves a two-stage frontal-chromatographic clean-up, followed by coupled minibore high-performance liquid chromatography with electrochemical detection. The use of phenylboronate instead of alumina yields recoveries in the range of 80–90% for all the catecholamines, dihydroxyphenylalanine and dihydroxyphenylacetic acid without interferences. The interassay coefficient of variation for the measurement of these compounds was less than 12% .