Striking paucity of HLA-A, B, C and beta 2-microglobulin on human neuroblastoma cell lines.
Striking paucity of HLA-A, B, C and beta 2-microglobulin on human neuroblastoma cell lines.
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DOI：
10.4049/jimmunol.130.5.2471
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发表时间：
1983-05
影响因子：
4.4
通讯作者：
L. Lampson;C. Fisher;J. Whelan
中科院分区：
文献类型：
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作者：
L. Lampson;C. Fisher;J. Whelan
使用β 2-微球蛋白（β 2m）单克隆抗体和天然双链分子单克隆抗体，评估HLA-A、B、C分子在人神经母细胞瘤来源细胞系上的表达。在使用新鲜细胞和固定细胞的放射免疫、细胞毒性和显微镜检测中，神经母细胞瘤细胞与胶质细胞或淋巴样细胞相比，充其量表现出较弱的活性。在结合抑制试验中，神经母细胞瘤提取物抑制抗体的效率比神经胶质或淋巴细胞提取物低200- 1800倍。免疫沉淀和SDS-PAGE分析证实，神经母细胞瘤细胞合成了β - m样链，但HLA链无法通过该技术可视化。已知HLA-A， B， C和β 2m水平在组织和细胞系之间存在差异。然而，神经母细胞瘤和淋巴细胞系之间的差异远远大于一些相同淋巴细胞系和许多其他非淋巴样恶性或非恶性细胞类型之间的表达差异。骨髓转移性神经母细胞瘤肿瘤也表现出较弱的HLA-A， B， C活性，细胞在显微镜下呈阴性。讨论了可能的临床意义。
Monoclonal antibodies to beta 2-microglobulin (beta 2m), and to the native two-chain molecule, were used to assess the expression of the HLA-A, B, C molecules on human neuroblastoma-derived cell lines. In radioimmuno-, cytotoxic, and microscopic assays, employing fresh and fixed cells, neuroblastoma cells show at best weak activity as compared to glial or lymphoid cells. In binding inhibition assays, neuroblastoma extracts were 200- to 1800-fold less efficient in inhibiting the antibodies than were glial or lymphoid extracts. Immunoprecipitation and SDS-PAGE analysis confirmed that a beta m-like chain is synthesized by the neuroblastoma cells, but the HLA chain could not be visualized by this technique. HLA-A, B, C and beta 2m levels are known to vary among tissues and cell lines. Yet the magnitude of the differences between the neuroblastoma and lymphoid lines is much greater than the reported differences in expression between some of these same lymphoid lines and many other nonlymphoid malignant or nonmalignant cell types. Metastatic neuroblastoma tumor in bone marrow also showed weak HLA-A, B, C activity, with the cells appearing negative in microscopic assays. Possible clinical implications are discussed.