Histochemical location of key enzyme activities involved in receptivity and self-incompatibility in the olive tree (Olea europaea L.)
Histochemical location of key enzyme activities involved in receptivity and self-incompatibility in the olive tree (Olea europaea L.)
复制标题
DOI：
10.1016/j.plantsci.2012.07.007
复制
发表时间：
2012-12-01
期刊：
影响因子：
5.2
通讯作者：
Olmedilla, Adela
中科院分区：
文献类型：
--
作者：
Serrano, Irene;Olmedilla, Adela
柱头和花柱表面酶对花粉的接受、选择和萌发具有重要作用。本报告涉及的组织化学定位的四种基本类型的酶参与这些过程中的橄榄（油橄榄）。柱头表面过氧化物酶、酯酶和酸性磷酸酶活性的检测为橄榄雌蕊的早期可授性提供了证据。柱头在花粉到达之前一直保持着可授性。酸性磷酸酶活性在开花时就开始在花柱中出现，并一直持续到胚珠受精。核糖核酸酶活性检测的花柱细胞外基质授粉前，并成为特别明显的雌蕊自花授粉后。这种活动逐渐减少，直到在更高级的阶段几乎消失。核糖核酸酶活性也检测到在授粉雌蕊中生长的花粉管中，并在自交不亲和雌蕊存在下的离体萌发后出现。这些结果表明，RNases可能参与了橄榄花的种内花粉排斥。据我们所知，这是第一次在本种中描述了柱头可授性和花粉选择中酶活性的证据。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Stigma-surface and style enzymes are important for pollen reception, selection and germination. This report deals with the histochemical location of the activity of four basic types of enzyme involved in these processes in the olive (Olea europaea). The detection of peroxidase, esterase and acid-phosphatase activities at the surface of the stigma provided evidence of early receptivity in olive pistils. The stigma maintained its receptivity until the arrival of pollen. Acid-phosphatase activity appeared in the style at the moment of anthesis and continued until the fertilization of the ovule. RNase activity was detected in the extracellular matrix of the styles of flowers just before pollination and became especially evident in pistils after self-pollination. This activity gradually decreased until it practically disappeared in more advanced stages. RNase activity was also detected in pollen tubes growing in pollinated pistils and appeared after in vitro germination in the presence of self-incompatible pistils. These findings suggest that RNases may well be involved in intraspecific pollen rejection in olive flowers. To the best of our knowledge this is the first time that evidence of enzyme activity in stigma receptivity and pollen selection has been described in this species. (C) 2012 Elsevier Ireland Ltd. All rights reserved.