Injectable and microporous scaffold of densely-packed, growth factor-encapsulating chitosan microgels.
Injectable and microporous scaffold of densely-packed, growth factor-encapsulating chitosan microgels.
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DOI：
10.1016/j.carbpol.2016.07.052
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发表时间：
2016-11-05
影响因子：
11.2
通讯作者：
Krebs MD
中科院分区：
文献类型：
--
作者：
Riederer MS;Requist BD;Payne KA;Way JD;Krebs MD
本研究采用乳化交联法制备了壳聚糖-京尼平微凝胶，并将其作为可注射的微孔支架材料。壳聚糖，其特征在于相对于pH值的光散射和水滴定。微凝胶的特征在于与溶胀，光散射，和流变学的密集包装的微凝胶解决方案。结果表明，随着壳聚糖变得越来越去质子化以上的pKa，排斥力减小和分子间的吸引力引起pH响应链聚集，导致微凝胶微凝胶聚集以及。具有最多壳聚糖和最少交联剂的微凝胶显示出最高的屈服应力和16 kPa的储能模量，当在pH 7.4下浓缩为微凝胶糊剂时。两种带相反电荷的生长因子可以被封装到微凝胶中，并且内皮细胞能够增殖到3D微凝胶支架中。这项工作激发了对壳聚糖微凝胶支架作为再生医学中可注射和微孔支架的应用的进一步研究。
In this work, an emulsion crosslinking method was developed to produce chitosan-genipin microgels which acted as an injectable and microporous scaffold. Chitosan was characterized with respect to pH by light scattering and aqueous titration. Microgels were characterized with swelling, light scattering, and rheometry of densely-packed microgel solutions. The results suggest that as chitosan becomes increasingly deprotonated above the pKa, repulsive forces diminish and intermolecular attractions cause pH-responsive chain aggregation; leading to microgel-microgel aggregation as well. The microgels with the most chitosan and least cross-linker showed the highest yield stress and a storage modulus of 16 kPa when condensed as a microgel paste at pH 7.4. Two oppositely-charged growth factors could be encapsulated into the microgels and endothelial cells were able to proliferate into the 3D microgel scaffold. This work motivates further research on the applications of the chitosan microgel scaffold as an injectable and microporous scaffold in regenerative medicine.