Small GTPase Cdc42 Is Required for Multiple Aspects of Dendritic Morphogenesis
Small GTPase Cdc42 Is Required for Multiple Aspects of Dendritic Morphogenesis
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DOI：
10.1523/jneurosci.23-08-03118.2003
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发表时间：
2003-04
期刊：
影响因子：
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通讯作者：
Ethan K. Scott;J. Reuter;L. Luo
中科院分区：
文献类型：
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作者：
Ethan K. Scott;J. Reuter;L. Luo
由于缺乏可以在易处理的遗传系统中研究的复杂树突结构，中枢神经系统神经元树突发育的研究受到了阻碍。为了开发这样的系统，我们最近对果蝇视觉系统中垂直系统（VS）神经元的高度复杂的树突进行了表征。使用 VS 神经元作为模型系统，我们在这里使用功能丧失突变表明，内源性 Cdc42（小 GTPase 的 Rho 家族成员）是树突形态发生的多个方面所必需的。Cdc42 突变的 VS 神经元表现出正常的复杂性，但与野生型相比，树突长度增加，并且在树突口径和定型树突分支位置方面存在缺陷。值得注意的是，Cdc42 突变神经元的树突棘密度也减少了 50%。这些结果表明 Cdc42 是树突发育多个方面的调节剂。
The study of dendritic development in CNS neurons has been hampered by a lack of complex dendritic structures that can be studied in a tractable genetic system. In an effort to develop such a system, we recently characterized the highly complex dendrites of the vertical system (VS) neurons in the Drosophila visual system. Using VS neurons as a model system, we show here using loss-of-function mutations that endogenous Cdc42, a member of Rho family of small GTPases, is required for multiple aspects of dendritic morphogenesis.Cdc42-mutant VS neurons display normal complexity but increased dendritic length compared with wild type and have defects in dendrite caliber and stereotyped dendritic branch positions. Remarkably, Cdc42 mutant neurons also show a 50% reduction in dendritic spine density. These results demonstrate that Cdc42 is a regulator for multiple aspects of dendritic development.