Phospholipase Cγ2 plays a role in TCR signal transduction and T cell selection.
Phospholipase Cγ2 plays a role in TCR signal transduction and T cell selection.
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DOI：
10.4049/jimmunol.1103458
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发表时间：
2012-09-01
期刊：
影响因子：
--
通讯作者：
Wen R
中科院分区：
文献类型：
--
作者：
Fu G;Chen Y;Schuman J;Wang D;Wen R
One of the important signaling events following T-cell receptor (TCR) engagement is activation of phospholipase Cγ (PLCγ). PLCγ has two isoforms, PLCγ1 and PLCγ2. It is known that PLCγ1 is important for TCR signaling and TCR-mediated T-cell selection and functions whereas PLCγ2 is critical for B-cell receptor (BCR) signal transduction and BCR-mediated B-cell maturation and functions. Here we report that PLCγ2 was expressed in primary T cells, and became associated with LAT and SLP-76 and activated upon TCR stimulation. PLCγ1/PLCγ2 double-deficient T cells displayed further block from CD4 and CD8 double positive to single positive transition compared to PLCγ1 single-deficient T cells. TCR-mediated proliferation was further impaired in PLCγ1/PLCγ2 double-deficient T cells compared PLCγ1 single-deficient T cells. TCR-mediated signal transduction, including Ca2+ mobilization and Erk activation, was further impaired in PLCγ1/PLCγ2 double-deficient relative to PLCγ1 single-deficient T cells. In addition, in HY TCR transgenic mouse model, thymic positive and negative selections were reduced in PLCγ1 heterozygous- and PLCγ2 homozygous-deficient (PLCγ1+/−PLCγ2−/−) relative to wild-type, PLCγ2 single-deficient (PLCγ2−/−), or PLCγ1 heterozygous-deficient (PLCγ1+/−) mice. Taken together, these data demonstrate that PLCγ2 participates in TCR signal transduction and plays a role in T-cell selection.
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影响因子：
3.5
作者：
HUANG, PS;DAVIS, L;HEIMBROOK, DC
通讯作者：
HEIMBROOK, DC
影响因子：
7.8
作者：
Page, D M;Kane, L P;Hedrick, S M
通讯作者：
Hedrick, S M
影响因子：
11.4
作者：
Falasca, M;Logan, SK;Schlessinger, J
通讯作者：
Schlessinger, J
DOI：
10.1046/j.1432-1327.1999.00560.x
发表时间：
1999-08-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Gross, BS;Melford, SK;Watson, SP
通讯作者：
Watson, SP
DOI：
10.1073/pnas.94.7.2999
发表时间：
1997-04-01
影响因子：
11.1
作者：
Ji, QS;Winnier, GE;Carpenter, G
通讯作者：
Carpenter, G