LINC01272 Promotes Migration and Invasion of Gastric Cancer Cells via EMT
LINC01272 Promotes Migration and Invasion of Gastric Cancer Cells via EMT
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DOI：
10.2147/ott.s242073
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发表时间：
2020-01-01
影响因子：
4
通讯作者：
Song, Fangzhou
中科院分区：
文献类型：
--
作者：
Leng, Xue;Liu, Geli;Song, Fangzhou
目的：胃癌是世界上第五大常见肿瘤，大多数胃癌患者预后差。本研究旨在探讨LINC 01272在胃癌中的生物学作用及其机制。材料与方法：采用生物信息学分析方法，对LINC 01272基因在胃癌中的表达进行分析，预测LINC 01272在胃癌中的生物学功能及其作用机制。采用实时荧光定量聚合酶链反应（real-time polymerase chain reaction，RT-PCR）检测LINC 01272在胃癌细胞株、胃癌组织及相应正常组织中的表达。结果：LINC 01272在胃癌组织中表达上调，并与胃癌分期和淋巴结转移有关。创伤愈合实验和Transwell实验结果显示LINC 01272与胃癌细胞的迁移和侵袭密切相关。LINC 01272基因敲低可通过降低EMT相关蛋白的表达抑制胃癌细胞的迁移和侵袭能力。结论：LINC 01272通过调控EMT相关蛋白的表达，促进胃癌转移能力，可作为胃癌诊断的潜在生物标志物。
Purpose: Gastric cancer (GC) is the fifth most common tumor in the world, and most patients with GC have a poor prognosis. This study aimed to explore the biological influence and mechanism of LINC01272 in GC.Materials and Methods: Using bioinformatic analyses, we investigated the expression of LINC01272 in TCGA database and predicted the biological functions and mechanism of LINC01272 in GC. Then, we detected the expression of LINC01272 in GC cell lines, GC tissues, and corresponding normal tissues using real-time polymerase chain reaction (RT-PCR). Finally, we explored the migration and invasion ability of LINC01272 by wound-healing and Transwell assays and examined the expression of epithelial-mesenchymal transition (EMT)related proteins through Western blotting.Results: We found that LINC01272 was upregulated in GC and was associated with GC staging and lymph node metastasis. The results of wound-healing and Transwell assays revealed that the LINC01272 was closely related to GC cell migration and invasion. LINC01272 knockdown inhibited the migration and invasion ability of GC cells by reducing the expression of EMT-related proteins. Overexpression of LINC01272 had the opposite effect.Conclusion: Together, our results showed that LINC01272 promoted GC metastasis ability by regulating the expression of EMT-related proteins and could serve as a potential diagnostic biomarker for GC.