Ultrafast 2D NMR spectroscopy using sinusoidal gradients: principles and ex vivo brain investigations.
Ultrafast 2D NMR spectroscopy using sinusoidal gradients: principles and ex vivo brain investigations.
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使用正弦梯度的超快 2D NMR 波谱：原理和离体大脑研究。
DOI：
10.1002/mrm.20204
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发表时间：
2004
影响因子：
3.3
通讯作者：
Frydman,Lucio
中科院分区：
文献类型：
--
作者：
Sela,Noa;Degani,Hadassa;Frydman,Lucio
最近介绍了一种能够在单次扫描内提供完整的2D NMR光谱的新方法。由此产生的时间分辨率的潜在增益可以打开新的机会，在体内光谱，提供的方法的技术要求是由相应的硬件满足。这些要求中最重要的是应用梯度回波序列所需的相对较短的切换时间。这些快速转变可能特别难以在成像系统上实现。作为解决该问题的一个步骤，我们评估了将采集过程中当前使用的方波梯度序列替换为成形正弦梯度的可能性。给出了该协议实施的示例，并演示了在显微成像探针上成功超快获取具有合适谱宽的2D NMR光谱（用于体模溶液和离体小鼠大脑）。Magn Reson Med 52：893-897，2004。© 2004 Wiley利斯公司
A new methodology capable of delivering complete 2D NMR spectra within a single scan was recently introduced. The resulting potential gain in time resolution could open new opportunities for in vivo spectroscopy, provided that the technical demands of the methodology are satisfied by the corresponding hardware. Foremost among these demands are the relatively short switching times expected from the applied gradient‐echo trains. These rapid transitions may be particularly difficult to accomplish on imaging systems. As a step toward solving this problem, we assessed the possibility of replacing the square‐wave gradient train currently used during the course of the acquisition by a shaped sinusoidal gradient. Examples of the implementation of this protocol are given, and successful ultrafast acquisitions of 2D NMR spectra with suitable spectral widths on a microimaging probe (for both phantom solutions and ex vivo mouse brains) are demonstrated. Magn Reson Med 52:893–897, 2004. © 2004 Wiley‐Liss, Inc.