Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Dierential Resistance to Salmonella
Transcriptome Analysis of the Cecal Tonsil of Jingxing Yellow Chickens Revealed the Mechanism of Dierential Resistance to Salmonella
复制标题
井陉黄鸡盲肠扁桃体转录组分析揭示沙门氏菌差异抗性机制
作者：
Fei Wang;Jin Zhang;Bo Zhu;JieWang;Qiao Wang;Maiqing Zheng;JieWen;Qinghe Li;Guiping Zhao
沙门氏菌是最常见的食源性致病菌之一。它可以在鸡之间传播，也可以通过受污染的家禽产品传播给人。在本研究中，我们主要根据细菌载量来区分不同抗性的鸡。我们比较了沙门氏菌感染后敏感和抗性鸡盲肠扁桃体转录组，旨在确定参与盲肠扁桃体抗菌活性的关键基因。在两组之间共鉴定出3214个差异表达基因（DEG），包括2092个上调基因和1122个下调基因（倍数变化>2.0，padj < 0.05）。许多DEG主要参与两个生物学过程的调节：盲肠扁桃体上皮和病原菌之间的串扰，如粘着斑，细胞外基质-受体相互作用，以及调节肌动蛋白细胞骨架和宿主免疫反应，包括精氨酸-受体相互作用。特别是，被挑战的抗性鸟表现出强烈的肠道免疫网络激活伊加的产生，这可能有助于抵抗沙门氏菌感染。这些研究结果提供了深入了解初始沙门氏菌刺激后两组之间的盲肠扁桃体的mRNA谱，这可能会扩展鸡对沙门氏菌免疫反应的分子机制的已知复杂性。
Salmonella is one of the most common food-borne pathogens. It can be transmitted between chickens, as well as to people by contaminated poultry products. In our study, we distinguished chickens with dierent resistances mainly based on bacterial loads. We compared the cecal tonsil transcriptomes between the susceptible and resistant chickens after Salmonella infection, aiming to identify the crucial genes participating in the antibacterial activity in the cecal tonsil. A total of 3214 differentially expressed genes (DEGs), including 2092 upregulated and 1122 downregulated genes, were identified between the two groups (fold change >2.0, padj < 0.05). Many DEGs were mainly involved in the regulation of two biological processes: crosstalk between the cecal tonsil epithelium and pathogenic bacteria, such as focal adhesion, extracellular-matrix–receptor interaction, and regulation of the actin cytoskeleton and host immune response including the cytokine–receptor interaction. In particular, the challenged resistant birds exhibited strong activation of the intestinal immune network for IgA production, which perhaps contributed to the resistance to Salmonella infection. These findings give insight into the mRNA profile of the cecal tonsil between the two groups after initial Salmonella stimulation, which may extend the known complexity of molecular mechanisms in chicken immune response to Salmonella.