Identification of Water Deficit Stress Upregulated Genes in Sugarcane
Identification of Water Deficit Stress Upregulated Genes in Sugarcane
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DOI：
10.1007/s11105-010-0230-0
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发表时间：
2011-06-01
影响因子：
2.1
通讯作者：
Prasad, Doddananjappa Theertha
中科院分区：
文献类型：
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作者：
Prabu, Gajjeraman;Kawar, Prashant Govindrao;Prasad, Doddananjappa Theertha
甘蔗是一种重要的可再生能源，在C4植物中具有很高的碳同化能力。由于淡水供应有限，这种作物不经济，因此有必要开发具有较高水分利用效率和耐缺水胁迫的品种。甘蔗品种Co 740经受不同水平的缺水胁迫以分离对所施加的胁迫差异表达的转录物。叶片相对含水量被用作估计胁迫响应的量度。应用基于PCR的cDNA抑制消减杂交技术构建了胁迫下差异表达基因的正向消减文库。斑点杂交选择158个克隆显示升高的反应进行测序，其中62%的相似性与已知的功能基因，12%与植物来源的假设蛋白质，而26%代表新的未知序列。这些差异表达的序列标签（EST）在中度水分亏缺胁迫耐受品种的注释预测，他们中的大多数编码的蛋白质参与细胞组织，蛋白质代谢，信号转导和转录。此外，半定量逆转录酶PCR进行了5个基因预计这些EST参与缓解/耐受压力。这项研究的结果可能有助于靶向有用的基因，以提高甘蔗和其他禾本科植物的耐旱性。
Sugarcane represents an important renewable source among biofuel crops with high capability to assimilate carbon among the C4 plants. Limited availability of freshwater renders this crop uneconomical, warranting the necessity for the development of varieties with higher water use efficiency and tolerant to water deficiency stress. Sugarcane variety cv. Co740 was subjected to varied levels of water deficiency stress to isolate transcripts differentially expressed to the imposed stress. The leaf relative water content was used as a measure to estimate the stress response. PCR-based cDNA suppression subtractive hybridization technique was applied to construct forward subtracted library for differentially expressed genes under stress. Dot blot-selected 158 clones showing elevated response were sequenced, of which 62% resembled similarity with known functional genes, 12% with hypothetical proteins of plant origin, while 26% represented new unknown sequences. Annotation of these differentially expressed sequence tags (ESTs) in the moderately water deficit stress-tolerant cultivar predicted that most of them encoded proteins involved in cellular organization, protein metabolism, signal transduction, and transcription. Further, semi-quantitative reverse transcriptase PCR carried out for five genes projected the involvement of these ESTs in stress alleviation/tolerance. Results from this study may help in targeting useful genes for improving drought tolerance in sugarcane and other grasses.