BIOLUMINESCENT SENSORS FOR DETECTION OF BIOAVAILABLE HG(II) IN THE ENVIRONMENT
BIOLUMINESCENT SENSORS FOR DETECTION OF BIOAVAILABLE HG(II) IN THE ENVIRONMENT
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DOI：
10.1128/aem.59.9.3083-3090.1993
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发表时间：
1993-09-01
影响因子：
4.4
通讯作者：
BARKAY, T
中科院分区：
文献类型：
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作者：
SELIFONOVA, O;BURLAGE, R;BARKAY, T
用于检测环境中污染物的生物传感器可以通过区分生物可用污染物和惰性不可用污染物来补充分析方法。通过将广为人知的TN21抗汞操纵子(MER)与来自费氏弧菌的无启动子LuxCDABE的融合，我们已经构建并测试了三种汞(II)生物传感器。由携带Merro/PT‘的pRB28、介导汞(II)主动转运的pOS14和含有完整的Mer操纵子的pOS15所指定的生物发光在丰富和极少量的介质中被测量。对pRB28、pOS14和pOS15的灵敏度最高，分别为1、0.5和25 nM汞(II)。用淡水、雨水和河口补充汞(II)的样品证明了该生物传感器在自然水域中的有效性。PRB28和pOS14携带的汞对从淡水池塘采集的汞污染水样中的汞(II)有反应。根据从池塘入口(经分析确定的总汞，约20 NM)和出水口(总汞，约7 NM)收集的样品的光发射进行的半定量分析显示，生物可用汞分别约为20和1至2 NM。因此，这里描述的生物传感器半定量地检测污染水中的生物可用无机汞(在纳摩尔到微摩尔浓度范围内)。
Biosensors for the detection of pollutants in the environment can complement analytical methods by distinguishing bioavailable from inert, unavailable forms of contaminants. By using fusions of the well-understood Tn21 mercury resistance operon (mer) with promoterless luxCDABE from Vibrio fischeri, we have constructed and tested three biosensors for Hg(II). Bioluminescence specified by pRB28, carrying merRo/pT', by pOS14, mediating active transport of Hg(II), and by pOS15, containing an intact mer operon, was measured in rich and minimal media. The highest sensitivities were achieved in minimal medium and were 1, 0.5, and 25 nM Hg(II) for pRB28, pOS14, and pOS15, respectively. The utility of the biosensors in natural waters was demonstrated with freshwater, rain, and estuarine samples supplemented with Hg(II). mer-lux carried by pRB28 and pOS14 responded to Hg(II) in mercury-contaminated water samples collected from a freshwater pond. Semiquantitative analyses based on light emission in samples collected from the inlet (analytically determined total mercury, approximately 20 nM) and outlet (total mercury, approximately 7 nM) of the pond showed bioavailable mercury at approximately 20 and 1 to 2 nM, respectively. Thus, the biosensors described here semiquantitatively detect bioavailable inorganic mercury (at a nanomolar to micromolar concentration range) in contaminated waters.