A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation
A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation
复制标题
DOI：
10.1038/ncb1991
复制
发表时间：
2009-12-01
影响因子：
21.3
通讯作者：
Yamamoto, Akitsugu
中科院分区：
文献类型：
--
作者：
Hayashi-Nishino, Mitsuko;Fujita, Naonobu;Yamamoto, Akitsugu
自噬是真核细胞中的大量降解过程，并且在细胞稳态中具有基本作用。自噬体膜的起源和来源是该领域的长期问题。使用电子显微镜，我们表明，在哺乳动物培养细胞中，内质网（ER）与早期的自噬结构相关称为分离膜（IMS）。 ATG4B突变体的过表达会导致自噬体形成缺陷，引起ER-IM复合物的积累。电子断层扫描显示，ER-IM复合物是ER的一个子域，形成了包围IM的摇篮，并表明ER和分离膜均相互联系。
Autophagy is a bulk degradation process in eukaryotic cells and has fundamental roles in cellular homeostasis. The origin and source of autophagosomal membranes are long-standing questions in the field. Using electron microscopy, we show that, in mammalian culture cells, the endoplasmic reticulum ( ER) associates with early autophagic structures called isolation membranes (IMs). Overexpression of an Atg4B mutant, which causes defects in autophagosome formation, induces the accumulation of ER-IM complexes. Electron tomography revealed that the ER-IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.