Wide variety of recombinant strains of norovirus GII in pediatric patients hospitalized with acute gastroenteritis in Thailand during 2005 to 2015
Wide variety of recombinant strains of norovirus GII in pediatric patients hospitalized with acute gastroenteritis in Thailand during 2005 to 2015
复制标题
2005年至2015年泰国急性胃肠炎住院儿科患者体内多种诺如病毒GII重组株
DOI：
10.1016/j.meegid.2017.04.025
复制
发表时间：
2017
影响因子：
3.2
通讯作者：
Maneekarn N
中科院分区：
文献类型：
--
作者：
Supadej K;Khamrin P;Kumthip K;Kochjan P;Yodmeeklin A;Ushijima H;Maneekarn N
诺如病毒（NoV）已被报道为全球儿童和成人急性胃肠炎的常见原因。在许多变体中，NoV GII.4是最主要的基因型。驱动NoV的新变体的进化和出现的机制之一是同源重组。本研究描述了2005年至2015年期间在泰国清迈急性胃肠炎儿科患者中检测到的NoV GII病例中涉及的基因重组。在总共1938份粪便样本中，3份（0.15%）为NoV GI阳性，298份（15.38%）被鉴定为NoV GII。本研究中检测到的基因型为GI.6、GI.14、GII.1、GII.2、GII.3、GII.4、GII.6、GII.7、GII.12、GII.13、GII.14、GII.15、GII.16、GII.17、GII.20和GII.21。通过ORF 1（RdRp）/ORF 2（衣壳）连接的部分序列分析，对NoV重组株进行了验证。ORF 1和ORF 2部分区域的系统发育分析导致21（6.98%）的重组株的鉴定。其中，本研究检测到9种重组模式; GII.Pe/GII.4、GII. Pg/GII.1、GII. Pg/GII.12、GII.P7/GII.6、GII.P7/GII.14、GII.P12/GII.4、GII.P16/GII.2、GII.P16/GII.13和GII.P21/GII.3。研究结果表明，在过去十年中，在泰国清迈因急性胃肠炎入院的儿科患者中检测到多种重组的NoV GII株。
Norovirus (NoV) has been reported as being a common cause of acute gastroenteritis both in children and adults worldwide. Of the many variants, NoV GII.4 is the most predominant genotype. One of the mechanisms that drives the evolution and emergence of new variants of NoV is homologous recombination. This study describes the genetic recombination involved in cases of NoV GII detected in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand during 2005 to 2015. From a total of 1938 stool samples, 3 (0.15%) were positive for NoV GI and 298 (15.38%) were identified as NoV GII. The genotypes detected in this study were GI.6, GI.14, GII.1, GII.2, GII.3, GII.4, GII.6, GII.7, GII.12, GII.13, GII.14, GII.15, GII.16, GII.17, GII.20, and GII.21. The NoV recombinant strains were verified by analysis of the partial sequence of ORF1 (RdRp)/ORF2 (capsid) junction. Phylogenetic analyses of partial ORF1 and ORF2 regions resulted in the identification of 21 (6.98%) NoV recombinant strains. Among these, 9 recombination patterns were detected in this study; GII.Pe/GII.4, GII.Pg/GII.1, GII.Pg/GII.12, GII.P7/GII.6, GII.P7/GII.14, GII.P12/GII.4, GII.P16/GII.2, GII.P16/GII.13, and GII.P21/GII.3. The findings demonstrated the wide variety of recombinant strains of NoV GII strains detected in pediatric patients admitted to the hospitals with acute gastroenteritis in Chiang Mai, Thailand during the past decade.