Receptor activator NFκB-ligand and osteoprotegerin protein expression in human periapical cysts and granulomas
Receptor activator NFκB-ligand and osteoprotegerin protein expression in human periapical cysts and granulomas
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DOI：
10.1016/j.tripleo.2005.10.054
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发表时间：
2006-09-01
期刊：
影响因子：
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通讯作者：
Granjeiro, Jose Mauro
中科院分区：
文献类型：
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作者：
Menezes, Renato;Bramante, Clovis Monteiro;Granjeiro, Jose Mauro
Objective.本研究的目的是确定与根尖周囊肿和肉芽肿中骨破坏相关的NF κ B B配体（RANKL）和骨保护素（OPG）的受体激活剂的表达。收集40例根尖周手术后的慢性根尖周病变。将收集的病变固定在10%缓冲福尔马林中并进行组织学处理。每份标本至少2张切片用苏木精和伊红染色，用于显微镜诊断。然后选取10例根尖肉芽肿和10例根尖囊肿进行RANKL、OPG和CD 68+的免疫组化检测。在两处病变中对多形性中性粒细胞、巨噬细胞、内皮细胞和淋巴细胞进行RANKL和OPG染色。根尖周囊肿中的上皮细胞也进行了RANKL和OPG染色。进行定量分析，结果表示为免疫染色细胞数与视野中细胞总数的比值（n = 100）。在根尖周肉芽肿（分别为0.553 +/- 0.153和0.483 +/- 0.189; P <0.0012;配对t检验）和囊肿（分别为0.519 +/- 0.09和0.339 +/- 0.117; P <0.0001;配对t检验）中，RANKL+/总细胞的比率高于OPG+/总细胞。肉芽肿中OPG+/总细胞（P <0.0001;配对t检验）和RANKL+/总细胞（P <0.0322;配对t检验）的比值大于囊肿。然而，肉芽肿（1.336 +/- 0.723）和囊肿（1.404 +/- 0.385）中的RANKL+/OPG（+）比值无显著差异。肉芽肿中CD 68 +/总细胞的比率（0.381 +/- 0.040）显著高于囊肿（0.307 +/- 0.068）（P <0.0001; Welch校正的非配对t检验）。考虑到所采用的实验方法的局限性，我们的研究结果表明RANKL和OPG存在于囊肿和肉芽肿中，强烈表明这些基因产物参与根尖周病变的发展。
Objective. The purpose of this study was to determine the expression of receptor activator of NF kappa B ligand (RANKL) and osteoprotegerin (OPG) associated with bone destruction in periapical cysts and granulomas.Study design. Forty human dental chronic periapical lesions were collected after periapical surgery. The lesions collected were fixed in 10% buffered formalin and histologically processed. At least 2 sections of each specimen were stained with hematoxylin and eosin for microscopic diagnosis. After that, 10 human periapical granulomas and 10 cysts were selected for immunohistochemical analysis for RANKL, OPG, and CD68+.Results. Polymorphonuclear neutrophils, macrophages, endothelial cells, and lymphocytes were stained for RANKL and OPG in both lesions. Epithelial cells were also stained for RANKL and OPG in periapical cysts. Quantitative analysis was conducted and the results were expressed as a ratio of the number of immunostained cells over the total number of cells in the field (n = 100). The ratio of RANKL+/total cells was higher than OPG+/total cells in periapical granulomas (0.553 +/- 0.153 and 0.483 +/- 0.189, respectively; P < .0012; paired t test) and in cysts (0.519 +/- 0.09 and 0.339 +/- 0.117, respectively; P < .0001; paired t test). The ratios of OPG+/total cells (P < .0001; paired t test) and RANKL+/total cells (P < .0322; paired t test) were greater in granulomas than in cysts. However, the ratio RANKL+/OPG(+) in granulomas (1.336 +/- 0.723) and cysts (1.404 +/- 0.385) was not significantly different. The ratio of CD68+/total cells was significantly higher in granulomas (0.381 +/- 0.040) than in cysts (0.307 +/- 0.068) (P < .0001; unpaired t test with Welch correction).Conclusion. Taking into account the limitations of the experimental approach employed, our findings indicate the presence of RANKL and OPG in cysts and granulomas, strongly suggesting the involvement of these gene products in the development of periapical lesions.