PROLIFERATIVE DEPENDENT REGULATION OF THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE URACIL DNA GLYCOSYLASE GENE IN HUMAN-CELLS
PROLIFERATIVE DEPENDENT REGULATION OF THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE URACIL DNA GLYCOSYLASE GENE IN HUMAN-CELLS
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DOI：
10.1093/carcin/13.11.2127
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发表时间：
1992-11-01
期刊：
影响因子：
4.7
通讯作者：
SIROVER, MA
中科院分区：
文献类型：
--
作者：
MEYERSIEGLER, K;RAHMANMANSUR, N;SIROVER, MA
在人细胞中检查了甘油醛-3-磷酸脱氢酶（GAPDH）/尿嘧啶DNA糖基化酶（UDG）基因的增殖依赖性表达与尿嘧啶DNA糖基化酶活性的诱导之间的关系。研究了三种不同的细胞类型，以确定这种多功能基因的生长依赖性调节是否是人类细胞的共同特征。这些细胞包括WI-38正常胚胎肺成纤维细胞、日本布卢姆综合征非转化皮肤成纤维细胞株（GM-05289）和由Epstein-巴尔病毒转化的类淋巴母细胞系。日本布卢姆氏综合征细胞显示与标记单克隆抗体40.10.09的免疫反应性改变，该标记单克隆抗体40.10.09表征来自这种人类遗传疾病的细胞。在非循环的人细胞中，使用含有人GAPDH/UDG cDNA的质粒（pChug 20.1）进行的北方印迹分析揭示了单个1.6kb转录物。在每种情况下，该基因的表达在细胞增殖期间增加。GAPDH/UDG基因表达的这种增加与UDG酶活性观察到的相同。此外，使用抗人UDG单克隆抗体，免疫反应性呈生长依赖性升高，表明抗原蛋白水平增加。这些结果表明：（i）GAPDH/UDG基因的表达依赖于细胞的增殖状态;和（ii）该基因的转录与尿嘧啶DNA糖基化酶活性水平之间存在相关性。
The relationship between the proliferative dependent expression of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)/uracil DNA glycosylase (UDG) gene and the induction of uracil DNA glycosylase activity was examined in human cells. Three different cell types were studied to determine whether the growth-dependent regulation of this multifunctional gene was a common characteristic of human cells. These included WI-38 normal embryonic lung fibroblasts, a Japanese Bloom's syndrome non-transformed skin fibroblast cell strain (GM-05289) and a lymphoblastoid cell line transformed by the Epstein - Barr virus. The Japanese Bloom's syndrome cells displayed the altered immunoreactivity with marker monoclonal antibody 40.10.09 which characterizes cells from this human genetic disorder. In non-cycling human cells Northern blot analysis using a plasmid (pChug 20.1) which contained the human GAPDH/UDG cDNA revealed a single 1.6 kb transcript. In each case, the expression of this gene was increased during cell proliferation. This increase in GAPDH/UDG gene expression was identical to that observed for UDG enzyme activity. Further, using anti-human UDG monoclonal antibodies, there was a growth-dependent rise in immunoreactivity suggesting an increase in the level of antigenic protein. These results demonstrate that: (i) the expression of the GAPDH/UDG gene was dependent on the proliferative state of the cell; and (ii) a correlation existed between the transcription of this gene and the level of uracil DNA glycosylase enzyme activity.