Effect of Carbendazim Resistance on Trichothecene Production and Aggressiveness of Fusarium graminearum
Effect of Carbendazim Resistance on Trichothecene Production and Aggressiveness of Fusarium graminearum
复制标题
多菌灵抗性对禾谷镰刀菌单端孢菌素产生和攻击性的影响
DOI：
10.1094/mpmi-22-9-1143
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发表时间：
2009-09-01
影响因子：
3.5
通讯作者：
Zhou, Ming-Guo
中科院分区：
文献类型：
--
作者：
Zhang, Yan-Jun;Yu, Jun-Jie;Zhou, Ming-Guo
禾谷镰刀菌（有性型，玉米赤霉）引起谷物的赤霉病，并用对人类和家养动物有害的多烯真菌毒素污染谷物。我国小麦赤霉病的防治主要依靠多菌灵，而我国小麦赤霉病的防治主要依靠对多菌灵的抗性。禾谷早熟禾现已广泛传播。评价了67株菌株在摇瓶培养或田间培养中的倍半萜生产。这些菌株包括60个野生型菌株（30个MBC抗性菌株和30个MBC敏感菌株）、3个β 2-微管蛋白密码子167处的MBC抗性定点突变体、3个β 2-微管蛋白密码子240处的MBC敏感定点突变体以及它们的MBC敏感野生型祖菌株ZF 21。感病小穗的发生率和F.还对所有菌株的田间谷粒中的禾谷镰刀菌DNA（AFgDNA）进行了评估。MBC抗性增加了摇瓶培养或田间生产中的二孢噻吩产量。虽然MBC抗性没有改变感染小穗的发生率，但它确实增加了AFgDNA。Tri 5基因表达增加的MBC耐药菌株在振荡培养。我们发现摇瓶培养中三烯产量和Tri 5基因表达之间存在显著的指数关系，田间籽粒中感染小穗或AFgDNA的发生率和三烯产量之间存在线性关系。
Fusarium graminearum (teleomorph, Gibberella zeae) causes head blight of cereals and contaminates grains with trichothecene mycotoxins that are harmful to humans and domesticated animals. Control of Fusarium head blight relies on carbendazim (MBC) in China, but resistance to MBC in F. graminearum is now widespread. Sixty-seven strains were evaluated for trichothecene production in shake culture or in the field. The strains included 60 wild-type strains (30 MBC-resistant and 30 MBC-sensitive), three MBC-resistant site-directed mutants at codon 167 in beta 2-tubulin, three MBC-sensitive site-directed mutants at codon 240 in beta 2-tubulin, and their MBC-sensitive wild-type progenitor strain ZF21. The incidence of infected spikelets and the amount of F. graminearum DNA in field grain (AFgDNA) also were evaluated for all strains. MBC resistance increased trichothecene production in shake culture or in the field. Although MBC resistance did not change the incidence of infected spikelets, it did increase AFgDNA. Tri5 gene expression increased in MBC-resistant strains grown in shake culture. We found a significant exponential relationship between trichothecene production and Tri5 gene expression in shake culture and a linear relationship between the incidence of infected spikelets or AFgDNA and trichothecene production in field grain.