Molecular diversity of astrocytes with implications for neurological disorders
Molecular diversity of astrocytes with implications for neurological disorders
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DOI：
10.1073/pnas.0402140101
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发表时间：
2004-06-01
影响因子：
11.1
通讯作者：
DePinho, RA
中科院分区：
文献类型：
--
作者：
Bachoo, RM;Kim, RS;DePinho, RA
星形胶质细胞代表了中枢神经系统中最丰富但了解最少的细胞类型。在这里，我们使用严格的实验策略，通过整合星形胶质细胞分化的几个体外模型系统、原代星形胶质细胞培养物和各种富含星形胶质细胞的中枢神经系统结构的微阵列数据集，从分子上定义星形胶质细胞谱系。星形胶质细胞数据集的交叉，加上非星形胶质细胞排除过滤器的应用，产生了许多星形胶质细胞特异性基因，这些基因具有显着不同的区域中枢神经系统表达模式。这些星形胶质细胞特异性基因的注释提供了星形胶质细胞谱系不同生理作用的直接分子记录。这种正常大脑的整体视角也为星形胶质细胞如何参与常见神经系统疾病（如阿尔茨海默病、帕金森病、中风、癫痫和原发性脑肿瘤）的发病机制提供了一个框架。
The astrocyte represents the most abundant yet least understood cell type of the CNS. Here, we use a stringent experimental strategy to molecularly define the astrocyte lineage by integrating microarray datasets across several in vitro model systems of astrocyte differentiation, primary astrocyte cultures, and various astrocyterich CNS structures. The intersection of astrocyte data sets, coupled with the application of nonastrocytic exclusion filters, yielded many astrocyte-specific genes possessing strikingly varied patterns of regional CNS expression. Annotation of these astrocyte-specific genes provides direct molecular documentation of the diverse physiological roles of the astrocyte lineage. This global perspective in the normal brain also provides a framework for how astrocytes may participate in the pathogenesis of common neurological disorders like Alzheimer's disease, Parkinson's disease, stroke, epilepsy, and primary brain tumors.