Untargeted metabolomic investigate milk and ruminal fluid of Holstein cows supplemented with Perilla frutescens leaf
Untargeted metabolomic investigate milk and ruminal fluid of Holstein cows supplemented with Perilla frutescens leaf
复制标题
对补充紫苏叶的荷斯坦奶牛的牛奶和瘤胃液进行非靶向代谢组学研究
DOI：
10.1016/j.foodres.2020.110017
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发表时间：
2021
影响因子：
8.1
通讯作者：
Yu Zhu
中科院分区：
文献类型：
--
作者：
Wang Bing;Sun Zhiqiang;Tu Yan;Si Bingwen;Liu Yunlong;Yang Lei;Luo Hailing;Yu Zhu
牛奶中的化合物对人体的营养需求和健康很重要。瘤胃代谢谱决定着饲粮营养，并决定着产奶量。紫苏叶（Perilla frutescen叶子，PFL）因其具有生物活性代谢物而成为一种常用的中药。本试验采用超高效液相色谱-四极杆飞行时间质谱联用技术，研究了PFL对饲喂基本全混合日粮（CON, n = 7）和每头奶牛添加300 g/d PFL （PFL, n = 7）的14头奶牛瘤胃液和乳两种生物体液代谢组的影响。乳PE-NMe (18:1（9Z)/18:1(9Z)）和DG （18:0/20:4(5Z、8Z、11Z、14Z)/0:0）、齐墩果酸和核苷酸上调，乳中链脂肪酸（2-羟基辛酸）下调。添加PFL增加了瘤胃液和乳汁中嘧啶核苷酸的丰度。瘤胃液和乳中嘧啶代谢和不饱和脂肪酸的生物合成途径丰富。乳汁2-羟基辛酸与瘤胃尿苷5-单磷酸正相关，与瘤胃脱氧胞苷负相关，胸苷与瘤胃二烯酸正相关。本研究发现，添加PFL可以通过调节嘧啶代谢和不饱和脂肪酸的生物合成途径，改变瘤胃代谢谱和乳合成。我们的新发现为瘤胃液和牛奶的代谢组学特征提供了全面的见解，支持了紫苏奶在奶牛中的潜在生产。
Milk compounds are important for human nutrient requirements and health. The ruminal metabolic profile is responsible for dietary nutrition and determines milk production.Perilla frutescensleaf (PFL) is a commonly used medicinal herb due to its bioactive metabolites. This study elucidated the effects of PFL on the metabolome of two biofluids (rumen fluid and milk) of 14 cows fed a basic total mixed ration diet (CON, n = 7) and supplemented with 300 g/d PFL per cow (PFL, n = 7) by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Milk PE-NMe (18:1(9Z)/18:1(9Z)) and DG (18:0/20:4(5Z,8Z,11Z,14Z)/0:0), oleanolic acid, and nucleotides were upregulated, and milk medium-chain fatty acids (2-hydroxycaprylic acid) were down-regulated in response to PFL. The supplementation of PFL increased the abundance of pyrimidine nucleotides both in rumen fluid and milk. The pathways of pyrimidine metabolism and biosynthesis of unsaturated fatty acids were enriched both in the rumen fluid and milk. We also found the milk 2-hydroxycaprylic acid was positively correlated with ruminal uridine 5-monophosphate, and was negatively correlated with ruminal deoxycytidine, and the milk thymidine was positively correlated with ruminal icosenoic acid. This study found that the supplementation of PFL could alter the ruminal metabolic profiles and milk synthesis through regulation of the pathways of pyrimidine metabolism and biosynthesis of unsaturated fatty acids. Our new findings provide comprehensive insights into the metabolomics profile of rumen fluid and milk, supporting the potential production ofPerilla frutescensmilk in dairy cows.