RNA-based analyses reveal fungal communities structured by a senescence gradient in the moss Dicranum scoparium and the presence of putative multi-trophic fungi
RNA-based analyses reveal fungal communities structured by a senescence gradient in the moss Dicranum scoparium and the presence of putative multi-trophic fungi
复制标题
DOI：
10.1111/nph.15092
复制
发表时间：
2018-06-01
期刊：
影响因子：
9.4
通讯作者：
Lutzoni, Francois
中科院分区：
文献类型：
--
作者：
Chen, Ko-Hsuan;Liao, Hui-Ling;Lutzoni, Francois
多种与植物相关的真菌被认为具有共生和腐生状态，因为它们可以从死亡和活的植物组织中分离出来。然而，这些组织通常在时间和空间上是分离的，并且在真菌群落研究中很少直接评估植物衰老各个阶段的真菌活性。我们使用真菌核糖体RNA宏转录组学来检测野生采集的麦角藻配子体（苔藓植物）内自然衰老梯度中的活跃真菌群落，以了解活跃真菌群落在邻近活组织、衰老组织和死亡组织中的分布。子囊菌在整个组织中的所有组织中都活跃。衰老梯度。相比之下，担子菌门在衰老和死亡的组织中普遍存在且活跃。在活组织和死组织中检测到几种真菌具有活性，表明它们具有多营养的能力。宏转录组学检测到的群落组装差异与 cDNA 扩增子测序相呼应，并与基于培养的推论和现场真菌子实体的观察进行比较。cDNA 扩增子测序和宏转录组学的结合有望用于研究具有复杂微生物多样性的共生系统，从而可以同时检测其存在和活性。
Diverse plant-associated fungi are thought to have symbiotrophic and saprotrophic states because they can be isolated from both dead and living plant tissues. However, such tissues often are separated in time and space, and fungal activity at various stages of plant senescence is rarely assessed directly in fungal community studies.We used fungal ribosomal RNA metatranscriptomics to detect active fungal communities across a natural senescence gradient within wild-collected gametophytes of Dicranum scoparium (Bryophyta) to understand the distribution of active fungal communities in adjacent living, senescing and dead tissues.Ascomycota were active in all tissues across the senescence gradient. By contrast, Basidiomycota were prevalent and active in senescing and dead tissues. Several fungi were detected as active in living and dead tissues, suggesting their capacity for multi-trophy. Differences in community assembly detected by metatranscriptomics were echoed by amplicon sequencing of cDNA and compared to culture-based inferences and observation of fungal fruit bodies in the field.The combination of amplicon sequencing of cDNA and metatranscriptomics is promising for studying symbiotic systems with complex microbial diversity, allowing for the simultaneous detection of their presence and activity.