Integrating histology and imaging mass spectrometry
Integrating histology and imaging mass spectrometry
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DOI：
10.1021/ac0351264
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发表时间：
2004-02-15
影响因子：
7.4
通讯作者：
Caprioli, RM
中科院分区：
文献类型：
--
作者：
Chaurand, P;Schwartz, SA;Caprioli, RM
MALDI（基质辅助激光解吸/电离）成像质谱（IMS）是一种新技术，可直接从薄组织切片表面生成肽和蛋白质信号的分子分布图和二维离子密度图。这允许获得关于蛋白质的相对丰度和空间分布的特定信息。其中一个重要方面是将这些特定离子图像与通过光学显微镜观察到的组织学特征相关联的机会。为了促进这一点，我们已经开发了协议，允许MALDI质谱成像和光学显微镜在同一部分进行。这些方案的关键组成部分涉及使用导电载玻片作为组织切片的样品支持物和MS友好组织染色方案。我们用蛋白质标准品和几种类型的组织切片证明了这些方法的有效性。尽管发生染色特异性强度变化，但染色和对照组织样本之间的整体蛋白质模式和光谱质量保持一致。此外，在染色切片上进行的成像质谱实验显示出良好的图像质量，其中由染色方案产生的蛋白质的离域最小。
MALDI (matrix-assisted laser desorption/ionization) imaging mass spectrometry (IMS) is a new technology that generates molecular profiles and two-dimensional ion density maps of peptide and protein signals directly from the surface of thin tissue sections. This allows specific information to be obtained on the relative abundance and spatial distribution of proteins. One important aspect of this is the opportunity to correlate these specific ion images with histological features observed by optical microscopy. To facilitate this, we have developed protocols that allow MALDI mass spectrometry imaging and optical microscopy to be performed on the same section. Key components of these protocols involve the use of conductive glass slides as sample support for the tissue sections and MS-friendly tissue staining protocols. We show the effectiveness of these with protein standards and with several types of tissue sections. Although stain-specific intensity variations occur, the overall protein pattern and spectrum quality remain consistent between stained and control tissue samples. Furthermore, imaging mass spectrometry experiments performed on stained sections showed good image quality with minimal delocalization of proteins resulting from the staining protocols.