PRR5, 7and 9 positively modulate TOR signaling-mediated root cell proliferation by repressing TANDEM ZINC FINGER 1 in Arabidopsis
PRR5, 7and 9 positively modulate TOR signaling-mediated root cell proliferation by repressing TANDEM ZINC FINGER 1 in Arabidopsis
复制标题
PRR5、7 和 9 通过抑制拟南芥中的 TANDEM ZINC FINGER 1 正向调节 TOR 信号传导介导的根细胞增殖
DOI：
10.1093/nar/gkz191
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发表时间：
2019-06-04
影响因子：
14.9
通讯作者：
Wang, Lei
中科院分区：
文献类型：
--
作者：
Li, Bin;Wang, Yan;Wang, Lei
生物钟协调许多植物生长和发育过程，包括下胚轴中的细胞伸长，它是否调节细胞增殖在很大程度上是未知的。在这里，我们已经发现，假反应调节（PRRs），昼夜核心振荡器的重要组成部分，影响根分生组织细胞增殖介导的雷帕霉素靶（TOR）信号。PRR的无效突变体显示出对糖激活的TOR信号传导的敏感性大大降低。我们随后确定了串联锌指1，编码加工体定位的RNA结合蛋白，作为介导TOR信号传导中PRR抑制的直接靶标。多条生化和遗传证据表明，TZF 1作用于PRR下游以减弱TOR信号传导。TZF 1可通过其串联锌指结构直接与TOR mRNA结合，影响TOR mRNA的稳定性。我们的研究结果支持了PRR-TZF 1-TOR分子轴通过整合转录和转录后调节机制来调节根分生组织细胞增殖的观点。
Circadian clock coordinates numerous plant growth and developmental processes including cell elongation in the hypocotyl, whether or not it modulates cell proliferation is largely unknown. Here we have found that Pseudo Response Regulators (PRRs), essential components of circadian core oscillators, affect root meristem cell proliferation mediated by Target Of Rapamycin (TOR) signaling. The null mutants of PRRs display much reduced sensitivities to sugar-activated TOR signaling. We have subsequently identified Tandem Zinc Finger 1, encoding a processing body localized RNA-binding protein, as a direct target repressed by PRRs in mediating TOR signaling. Multiple lines of biochemical and genetic evidence have demonstrated that TZF1 acts downstream of PRRs to attenuate TOR signaling. Furthermore, TZF1 could directly bind TOR mRNA via its tandem zinc finger motif to affect TOR mRNA stability. Our findings support a notion that PRR-TZF1-TOR molecular axis modulates root meristem cell proliferation by integrating both transcriptional and post-transcriptional regulatory mechanisms.