Advances on the interaction of polypyridyl Cr(III) complexes with transporting proteins and its potential relevance in photodynamic therapy.
Advances on the interaction of polypyridyl Cr(III) complexes with transporting proteins and its potential relevance in photodynamic therapy.
复制标题
聚吡啶 Cr(III) 复合物与转运蛋白相互作用的进展及其在光动力治疗中的潜在相关性。
DOI：
10.1016/j.jinorgbio.2011.07.013
复制
发表时间：
2011
影响因子：
3.9
通讯作者：
G. Argüello
中科院分区：
文献类型：
--
作者：
J. Toneatto;P. F. Garcia;G. Argüello
本研究详细研究了 [Cr(phen)2(dppz)]3+ 和 [Cr(phen)3]3+ 与转铁蛋白（血浆中转运 Fe3+ 的关键蛋白）的相互作用；它的循环有望成为针对肿瘤组织的药物靶向策略的有吸引力的系统。这可以让我们进一步了解这两种复合物作为光动力疗法（PDT）中敏化剂的作用。铬 (III) 络合物 [Cr(phen)2(dppz)]3+ 和 [Cr(phen)3]3+（phen=1,10-菲咯啉和 dppz=联吡啶吩嗪），其中 dppz 是具有扩展 π 系统的平面二齿配体，已被发现与脱铁转铁蛋白 (apoTf) 牢固结合，其内在结合常数 Kb 为299K 时，apoTf–[Cr(phen)2(dppz)]3+ 和 apoTf–[Cr(phen)3]3+ 分别为 (1.8±0.3)×105M−1 和 (1.1±0.1)×105M−1。采用紫外可见吸收、荧光和圆二色光谱评估了 apoTf 与不同 Cr(III) 配合物的相互作用。随着 Cr(III) 复合物浓度的增加，蛋白质的相对荧光强度降低，表明色氨酸和酪氨酸残基周围发生了扰动。热力学参数 ΔG、ΔH、ΔS 的分析表明 Cr(III) 复合物的存在稳定了蛋白质，并具有很强的熵贡献。根据Föster非辐射能量转移理论计算apoTf–[Cr(phen)2(dppz)]3+和apoTf–[Cr(phen)3]3+结合反应的结合距离和转移效率。所有这些实验结果表明[Cr(phen)2(dppz)]3+和[Cr(phen)3]3+与apoTf强烈结合，表明该蛋白可以作为这些复合物的载体在PDT中进一步应用。
The present study reports a detailed investigation into the interaction of [Cr(phen)2(dppz)]3+and [Cr(phen)3]3+with transferrin, the key protein for the transport of Fe3+in blood plasma; its cycle holds promise as an attractive system for strategies of drug targeting to tumor tissues. This can allow us to understand further the role of both complexes as sensitizers in photodynamic therapy (PDT). Chromium(III) complexes, [Cr(phen)2(dppz)]3+and [Cr(phen)3]3+, (phen=1,10-phenanthroline and dppz=dipyridophenazine), where dppz is a planar bidentate ligand with an extended π system, have been found to bind strongly with apotransferrin (apoTf) with an intrinsic binding constant, Kb, of (1.8±0.3)×105M−1and (1.1±0.1)×105M−1at 299K, for apoTf–[Cr(phen)2(dppz)]3+and apoTf–[Cr(phen)3]3+, respectively. The interactions of apoTf with the different Cr(III) complexes were assessed employing UV–visible absorption, fluorescence and circular dichroism spectroscopy. The relative fluorescence intensity of the protein decreased when the increasing concentration of Cr(III) complex was added, suggesting that perturbation around the Trp and Tyr residues took place. The analysis of the thermodynamic parameters ΔG, ΔH, ΔS indicated that the presence of the Cr(III) complex stabilizes the protein with a strong entropic contribution. The binding distances and transfer efficiencies for apoTf–[Cr(phen)2(dppz)]3+and apoTf–[Cr(phen)3]3+binding reactions were calculated according to Föster theory of non-radiation energy transfer. All these experimental results suggest that [Cr(phen)2(dppz)]3+and [Cr(phen)3]3+bind strongly to apoTf indicating that this protein could act as a carrier of these complexes for further applications in PDT.
影响因子：
3.9
作者：
Martinez, Alberto;Suarez, Javier;Shand, Tiffany;Magliozzo, Richard S.;Sanchez-Delgado, Roberto A.
通讯作者：
Sanchez-Delgado, Roberto A.
影响因子：
3.9
作者：
BAKER, EN;LINDLEY, PF
通讯作者：
LINDLEY, PF