Citric acid-derived in situ crosslinkable biodegradable polymers for cell delivery
Citric acid-derived in situ crosslinkable biodegradable polymers for cell delivery
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DOI：
10.1016/j.biomaterials.2010.08.022
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发表时间：
2010-12-01
期刊：
影响因子：
14
通讯作者：
Yang, Jian
中科院分区：
文献类型：
--
作者：
Gyawali, Dipendra;Nair, Parvathi;Yang, Jian
在此，我们报道了第一个柠檬酸（CA）衍生的原位交联可生物降解聚合物，聚乙二醇马来酸柠檬酸酯（PEGMC）。PEGMC的合成可以在不使用有机溶剂和催化剂的情况下通过一锅缩聚反应进行。聚乙二醇甲基丙烯酸酯可以原位交联成弹性聚乙二醇甲基丙烯酸酯水凝胶。水凝胶在溶胀、降解和力学性能方面的性能高度依赖于合成过程中单体的摩尔比、交联剂的浓度和交联机理。循环调节试验表明，PPEGMC水凝胶可以被压缩到75%的应变而不产生永久变形，并且可以忽略迟滞。水溶性PEGMC在体外表现出良好的细胞相容性。PPEGMC的降解产物在体外也表现出极小的细胞毒性。大鼠动物实验清楚地证明了PEGMC良好的注射性和原位形成的PEGMC的可降解性。PPEGMC在注射后的早期阶段引起最小的炎症，并在30天内完全降解。总之，ca衍生的可注射可生物降解PEGMC的发展为材料创新提供了许多机会，并为原位组织工程和药物输送应用提供了优秀的候选材料。(C) 2010 Elsevier Ltd.版权所有。
Herein, we report a first citric acid (CA)-derived in situ crosslinkable biodegradable polymer, poly (ethylene glycol) maleate citrate (PEGMC). The synthesis of PEGMC could be carried out via a one-pot polycondensation reaction without using organic solvents or catalysts. PEGMC could be in situ crosslinked into elastomeric PPEGMC hydrogels. The performance of hydrogels in terms of swelling, degradation, and mechanical properties were highly dependent on the molar ratio of monomers, crosslinker concentration, and crosslinking mechanism used in the synthesis process. Cyclic conditioning tests showed that PPEGMC hydrogels could be compressed up to 75% strain without permanent deformation and with negligible hysteresis. Water-soluble PEGMC demonstrated excellent cytocompatibilty in vitro. The degradation products of PPEGMC also showed minimal cytotoxicity in vitro. Animal studies in rats clearly demonstrated the excellent injectability of PEGMC and degradability of the in situ-formed PPEGMC. PPEGMC elicited minimal inflammation in the early stages post-injection and was completely degraded within 30 days in rats. In conclusion, the development of CA-derived injectable biodegradable PEGMC presents numerous opportunities for material innovation and offers excellent candidate materials for in situ tissue engineering and drug delivery applications. (C) 2010 Elsevier Ltd. All rights reserved.